| 喂食当归多糖对点带石斑鱼非特异性免疫力和抗病力的影响 |
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| 引用本文： | 王庆奎,邢克智,赵海运,陈成勋,毋占勇,马甡.喂食当归多糖对点带石斑鱼非特异性免疫力和抗病力的影响[J].中国海洋大学学报(自然科学版),2012(9):15-21. |
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| 作者姓名： | 王庆奎 邢克智 赵海运 陈成勋 毋占勇 马甡 |
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| 作者单位： | 中国海洋大学海水养殖教育部重点实验室;天津农学院天津市水产生态及养殖重点实验室 |
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| 基金项目： | “十二五”国家科技支撑计划项目(2011BAD13B07)资助 |
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| 摘 要： | 在饵料中添加0、500、3 000mg/kg的当归多糖(ASP)喂食点带石斑鱼(Epinephelus malabaricus)(初始体重(158.64±2.14)g),分别在喂食4和8周后取样,研究ASP对点带石斑鱼非特异性免疫力和抗病力的影响。结果表明,ASP能显著提高血液白细胞吞噬率和血清LZM活力(P<0.05),对血清一氧化氮(NO)、体表黏液溶菌酶(LZM)活力和抗菌活力无显著影响。ASP能促进头肾白细胞增殖、呼吸爆发活力和吞噬活力,降低用迟缓爱德华菌(Edwardsiella tar-da)攻毒后试验鱼的累积死亡率,3 000mg/kg组累积死亡率低于500mg/kg组,连续喂食ASP 8周的保护效果优于4周。研究表明,用含ASP 3 000mg/kg的饵料连续喂食点带石斑鱼8周能有效提高其非特异性免疫力和抗病力。
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| 关 键 词： | 当归多糖 点带石斑鱼 非特异性免疫力 抗病力 |
Dietary Angelica sinensis polysaccharide on Innate Immunity and Disease Resistance Against Edwardsiella tarda of Epinephelus malabaricus |
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| WANG Qing-Kui,XING Ke-Zhi,ZHAO Hai-Yun,CHEN Cheng-Xun,WU Zhan-Yong,MA.Dietary Angelica sinensis polysaccharide on Innate Immunity and Disease Resistance Against Edwardsiella tarda of Epinephelus malabaricus[J].Periodical of Ocean University of China,2012(9):15-21. |
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| Authors: | WANG Qing-Kui XING Ke-Zhi ZHAO Hai-Yun CHEN Cheng-Xun WU Zhan-Yong MA |
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| Institution: | Shen1(1.The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China;2.Tianjin Key Laboratory of Aqua-Ecology and Aquaculture,Tianjin Agricultural University,Tianjin 300384,China) |
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| Abstract: | In order to study the effects of Angelica sinensis polysaccharide(ASP) on innate immunity and disease resistance of Epinephelus malabaricus,fish(initial body weight(158.64±2.14) g) were fed diets containing different doses of ASP(0,500,3000 mg/kg) for four and eight weeks.Results showed that dietary ASP enhanced blood leucocytes phagocytic rate and serum lysozyme activity significantly(P<0.05),but exerted no influence on serum nitric oxide(NO),skin mucus lysozyme activity and antibacterial activity.Dietary ASP boosted head kidney leucocytes proliferation,respiratory burst activity and phagocytosis.The cumulative mortalities of fish challenged by Edwardsiella tarda were reduced in ASP-fed groups,compared to that in control groups.The cumulative mortality in fish fed with ASP at 3000 mg /kg for eight weeks was the lowest.The innate immunity and disease resistance against E.tarda in fish fed ASP at 3000 mg/kg for eight weeks were effectively enhanced. |
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| Keywords: | Angelica sinensis polysaccharide Epinephelus malabaricus innate immunity antibacterial activity |
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