| 鲢序列已知标记连锁图构建及鲢-草鱼标记共线性分析 |
| |
| 引用本文： | 杨静,张鹏,危起伟,邹桂伟,杨官品.鲢序列已知标记连锁图构建及鲢-草鱼标记共线性分析[J].中国海洋大学学报(自然科学版),2012(Z2):76-81. |
| |
| 作者姓名： | 杨静 张鹏 危起伟 邹桂伟 杨官品 |
| |
| 作者单位： | 中国海洋大学海洋生物遗传育种教育部重点实验室;中国水产科学研究院长江水产研究所 |
| |
| 基金项目： | 现代农产品加工技术研发体系专项基金项目(nycytx-49-01);农业部淡水鱼类种质资源与生物技术重点开放实验室开放课题(LFB20070601)资助 |
| |
| 摘 要： | 鲢、草鱼是传统池塘养殖的四大家鱼的2个重要成员,其遗传资源评估与管理、性状遗传基础研究、功能基因从头克隆等迫切需要序列已知标记连锁图谱工具。本研究将已有鲢连锁图谱新整合53个草鱼微卫星DNA标记和28个鲢InDel-RFLP标记,构建了鲢已知序列标记连锁图谱,标记数326个,达到中等密度水平。构建的图谱将为鲢、鳙资源评价和管理、性状遗传基础研究等提供工具。另外,本研究初步比较了鲢、草鱼间标记共线性排列,发现两物种染色体既存在共线性,也存在明显重排。
|
| 关 键 词： | 鲢 草鱼 序列已知标记 微卫星DNA标记 连锁图谱 标记共线性 |
Construction of a Sequence-known Marker Linkage Map of Silver Carp and the Marker Synteny Comparison between Silver Carp and Grass Carp |
| |
| YANG Jing,ZHANG Peng,WEI Qi-Wei,ZOU Gui-Wei,YANG Guan-Pin.Construction of a Sequence-known Marker Linkage Map of Silver Carp and the Marker Synteny Comparison between Silver Carp and Grass Carp[J].Periodical of Ocean University of China,2012(Z2):76-81. |
| |
| Authors: | YANG Jing ZHANG Peng WEI Qi-Wei ZOU Gui-Wei YANG Guan-Pin |
| |
| Institution: | 1(1.The Key Laboratory of Marine Genetics and Breeding,Ministry of Education,Ocean University of China,Qingdao 266003,China;2.Yangtze River Fisheries Research Institute,Chinese Academy of Fishery,Wuhan 430223,China) |
| |
| Abstract: | Silver carp and grass carp are two important members of the traditionally pond-cultured four carps with the most important pond-cultured fish species.An appropriate molecular marker linkage map is urgently needed for the evaluation and management of their genetic resources,the exploitation of the genetic bases of their important traits and de novo cloning of their functional genes.In this study,a midium-density sequence-known marker linkage map of silver carp was constructed by integrating 53 microsatellite DNA markers on the linkage map of grass carp and 28 InDel-RFLP markers developed for silver carp into the linkage map of silver carp constructed previously,on which the number of markers reached 326.The map will serve as a tool for the evaluation and management of their genetic resources and the exploitation of the genetic bases of traits.In addition,a preliminary comparison of marker synteny was made between grass carp and silver carp with obvious collinear arrangement and rearrangement of markers between two species identified. |
| |
| Keywords: | hypophthalmichthys molitrix ctenopharyngodon idella sequence-based marker microsatellite DNA marker linkage map marker synteny |
| 本文献已被 CNKI 等数据库收录！ |
|
