Well che89, located in the Chepaizi area in the northwest margin of Junggar basin, acquires high production industrial oil flow, which is an important breakthrough in the exploration of the south foreland slope area of Junggar basin. The Chepaizi area is near two hydrocarbon generation depressions of Sikeshu and Shawan, which have sets of hydrocarbon source rock of Carboniferous to Jurassic as well as Upper Tertiary. Geological and geochemical parameters are proper for the accumulation of mixed source crude oil. Carbon isotope, group composition and biomarkers of crude oil in Upper Tertiary of well Che89 show that the features of crude oil in Upper Tertiary Shawan Formation are between that of Permian and Jurassic, some of them are similar to these two, and some are of difference, they should be the mixed source of Permian and Jurassic. Geochemical analysis and geological study show that sand extract of Lower Tertiary Wulunguhe Formation has the same source as the crude oil and sand extract of Upper Tertiary Shawan Formation, but they are not charged in the same period. Oil/gas of Wulunguhe Formation is charged before Upper Tertiary sedimentation, and suffered serious biodegradation and oxidation and rinsing, which provide a proof in another aspect that the crude oil of Upper Tertiary Shawan Formation of well Che89 is not from hydrocarbon source rock of Lower Tertiary.