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| 青虾(Macrobrachium nipponense)Kazal型丝氨酸蛋白酶抑制因子基因全长cDNA的克隆及表达 | |
| 引用本文： | 张 响,乔 慧,傅洪拓,吴 滟,龚永生,蒋速飞,熊贻伟.青虾(Macrobrachium nipponense)Kazal型丝氨酸蛋白酶抑制因子基因全长cDNA的克隆及表达[J].海洋与湖沼,2012,43(6):1292-1298. |
| 作者姓名： | 张 响 乔 慧 傅洪拓 吴 滟 龚永生 蒋速飞 熊贻伟 |
| 作者单位： | 1. 南京农业大学渔业学院,无锡,214081 2. 中国水产科学研究院淡水渔业研究中心农业部淡水渔业和种质资源利用重点实验室,无锡,214081 3. 南京农业大学渔业学院,无锡214081 中国水产科学研究院淡水渔业研究中心农业部淡水渔业和种质资源利用重点实验室,无锡214081 |
| 基金项目： | 国家“十二五”科技支撑计划, 2012BAD26B04号, 2012BAD25B07号; 农业科技成果转化资金项目, 2010GB23260592号; 江苏省科技支撑计划, BE2010368号; 中央级基本科研业务费专项, 2011JBFA02号, 2011JBFC01号。 |
| 摘 要： | 采用RACE技术,首次从青虾精巢中克隆得到KSPI基因全长cDNA。青虾KSPI基因cDNA全长890bp,包括80bp的5′UTR,546bp的3′UTR和编码87个氨基酸残基的264bp开放阅读框。预测蛋白包含1个保守的Kazal型结构域(CⅠX5CⅡX(6)VCⅢX(5)TYXNXCⅣX6CⅤX12CⅥ),结构域中P1活性位点为苏氨酸残基。应用MEGA4.1软件对青虾与已报道的虾类共15种KSPI氨基酸序列进行系统进化分析,结果表明,青虾KSPI与同样来源于精巢的罗氏沼虾KSPI进化关系最近聚为一支,其它虾类来源于肝胰腺和血细胞的KSPI聚为另外两支。采用定量PCR技术分析其组织分布,结果显示,KSPI基因在精巢、心脏和卵巢中有较高表达,其中精巢表达水平极高,并与心脏和卵巢表达差异分别达到显著(P<0.05)和极显著(P<0.01)水平,其它组织表达较弱。 |
| 关 键 词： | 日本沼虾 青虾 精巢 丝氨酸蛋白酶抑制因子 cDNA 定量PCR |
| 收稿时间： | 2012/2/28 0:00:00 |
| 修稿时间： | 2012/4/19 0:00:00 |
THE FULL LENGTH cDNA CLONING AND EXPRESSION ANALYSIS OF KAZAL-TYPE SERINE PROTEINASE INHIBITOR GENE FROM MACROBRACHIUM NIPPONENSE |
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| ZHANG Xiang,QIAO Hui,FU Hong-Tuo,WU Yan,GONG Yong-Sheng,JIANG Su-Fei and XIONG Yi-Wei.THE FULL LENGTH cDNA CLONING AND EXPRESSION ANALYSIS OF KAZAL-TYPE SERINE PROTEINASE INHIBITOR GENE FROM MACROBRACHIUM NIPPONENSE[J].Oceanologia Et Limnologia Sinica,2012,43(6):1292-1298. | |
| Authors: | ZHANG Xiang QIAO Hui FU Hong-Tuo WU Yan GONG Yong-Sheng JIANG Su-Fei and XIONG Yi-Wei |
| Institution: | Wuxi Fishery College, Nanjing Agricultural University;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;Wuxi Fishery College, Nanjing Agricultural University; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences |
| Abstract: | |
| Keywords: | Macrobrachium nipponense Oriental river prawn Testis Serine proteinase inhibitor cDNA Quantitative PCR |
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