Degradation and utilization of protein derived from <Emphasis Type="Italic">Pseudomonas aeruginosa</Emphasis> by marine microbial community |
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| Authors: | Yumiko Obayashi Nahomi Ueoka Satoru Suzuki |
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| Institution: | (1) Lenox Institute of Water Technology, Lenox, MA, USA;(2) Krofta Engineering Corporation, Lenox, MA, USA;(3) Zorex Corporation, Newtonville, NY, USA;(4) Rensselaer Polytechnic Institute, Clifton Park, NY, USA; |
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| Abstract: | Microbial degradation and utilization of proteins derived from bacterial detritus were investigated in a microcosm experiment
using Pseudomonas aeruginosa detritus as a substrate. To assess the effects of natural marine microbial communities on degradation and utilization of
protein derived from P. aeruginosa cells, four microcosms were prepared: natural seawater (containing the natural microbial community) with P. aeruginosa detritus (N+Pa), autoclaved seawater with P. aeruginosa detritus (A+Pa), natural seawater (N) and autoclaved seawater (A) without adding anything as a control. The numbers of total
and growing bacterial cells, protease activity, and transition of P. aeruginosa proteins were monitored in the four microcosms. Changes in the numbers of total and growing bacterial cells and protease
activities indicated that bacterial detritus significantly stimulated the microbial community in the microcosms. Both the
surviving P. aeruginosa in A+Pa and natural microbial community in N+Pa microcosms were able to degrade and utilize P. aeruginosa detritus; however, the community in N+Pa including various microbes maintained high activity longer, indicating that diversity
is an important factor in keeping the community active. Even under the very high protease activity in N+Pa, 39-kDa and 48-kDa
proteins from P. aeruginosa remained in the microcosm during the entire experiment (150 days). Immunoblotting suggested the 48-kDa protein was an intact
molecule of OprP, which had been detected from the dissolved fraction of natural seawater in previous studies. This result
suggests that the protein molecules that had been detected from natural seawater actually had a high tolerance to microbial
degradation. |
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