| The influence of Fe^2＋ on growth and development of cells enzymatically isolated from Porphyra yezoensis blades |
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| 引用本文： | 沈颂东,戴继勋.The influence of Fe^2＋ on growth and development of cells enzymatically isolated from Porphyra yezoensis blades[J].中国海洋与湖沼学报,2005,23(1):48-53. |
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| 作者姓名： | 沈颂东 戴继勋 |
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| 作者单位： | [1]Department of Biology, College of Life Sciences, Suzhou University, Suzhou 215006, China [2]College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003, China |
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| 基金项目： | This work was supported by the NSFC （No. 40206019）. |
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| 摘 要： | Fe^2＋ acted as an accessorial factor for many cellular enzymatic reactions is very important for seaweed growth and development, but the Fe^2＋ requirement in nori had not been seen. Porphyra yezoensis cells were separated enzymatically and cultured in a series of sterilized seawater media containing various concentrations of Fe^2＋. The growth development and cell were investigated in this work. Through this experiment, two biologically-meant concentration scales were found, one is low concentrations, 12.1-102.1μg/L, 10-100 times than that in seawater, favoring the development of isolated cells of Porphyra and the other was high concentrations, more than 10mg/L inhibiting the cell growth, leading to the deformity and shrinkage of the cells. At the concentration of 50 mg/L, the cells stopped growing and died eventually.
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| 关 键 词： | Fe^2＋ 海水 分离工艺 海洋化学 |
| 收稿时间： | 2003-03-12 |
| 修稿时间： | 2004-01-29 |
The influence of Fe2+ on growth and development of cells enzymatically isolated fromPorphyra yezoensis blades |
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| Shen Songdong,Dai Jixun.The influence of Fe2+ on growth and development of cells enzymatically isolated fromPorphyra yezoensis blades[J].Chinese Journal of Oceanology and Limnology,2005,23(1):48-53. |
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| Authors: | Shen Songdong Dai Jixun |
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| Institution: | (1) Department of Biology, College of Life Sciences, Suzhou University, 215006 Suzhou, China;(2) College of Marine Life Sciences, Ocean University of Qingdao, 266003 Qingdao, China |
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| Abstract: | Fe2+ acted as an accessorial factor for many cellular enzymatic reactions is very important for seaweed growth and development,
but the Fe2+ requirement in nori had not been seen,Porphyra yezoensis cells were separated enzymatically and cultured in a series of sterilized seawater media containing various concentrations
of Fe2+. The growth development and cell were investigated in this work. Through this experiment, two biologically-meant concentration
scales were found, one is low concentrations, 12.1–102.1 μg/L, 10–100 times than that in seawater, favoring the development
of isolated cells ofPorphyra and the other was high concentrations, more than 10mg/L inhibiting the cell growth, leading to the deformity and shrinkage
of the cells. At the concentration of 50 mg/L, the cells stopped growing and died eventually.
This work was supported by the NSFC (No. 40206019). |
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| Keywords: | Fe2+ Porphyra yezoensis cell culture growth development |
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