| 马氏珠母贝育珠细胞小片和珠核处理技术研究Ⅱ:不同处理液在广东徐闻的育珠效果 |
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| 引用本文： | 王梅芳,余祥勇,李咏梅,宾承勇,豆伟,万正平,陈晓汉.马氏珠母贝育珠细胞小片和珠核处理技术研究Ⅱ:不同处理液在广东徐闻的育珠效果[J].广东海洋大学学报,2010,30(6). |
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| 作者姓名： | 王梅芳 余祥勇 李咏梅 宾承勇 豆伟 万正平 陈晓汉 |
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| 作者单位： | 1. 广东海洋大学水产学院,南海水产经济动物增养殖广东普通高校重点实验室,广东,湛江,524025
2. 广西水产研究所,广西,南宁,530021 |
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| 摘 要： | 通过海区插核育珠试验,对不同处理液匹配组进行进一步优化、筛选,找出适于广东育珠海区的育贝小片处理液及与之匹配的珠核处理液。结果表明:1)匹配适宜的处理液有降低吐核率的作用,8个试验小组中W0、Y3组的吐核率仅31%~32%,明显低于对照组的46.2%(P<0.01),说明匹配液有明显增加组织亲和力、降低吐核率的效果;2)育珠贝的死亡主要发生在育珠前期,与贝的吐核行为有关;3)小片处理液中,C类药物成分对吐核率的高低有影响,0.6%C2不适宜添加到处理液中,而1%C3添加到处理液中有降低吐核率的效果;4)筛选出W0、W1、Y3三组适宜的匹配方案,即W0、W1、Y3三种小片处理液与核处理液C3匹配适宜,其中W0匹配组育珠效果最佳,即成活率为57.8%,百贝收珠数为101.2粒、优珠率为67.5%,比传统处理组(对照组)有明显提高;5)对W0、W1、Y3三组的不同育珠时限(8、10、13个月)的育珠情况进行了比较,当育珠期延长至1年时,Y3组育珠效果良好;6)利用评价指数(即优质珍珠数占手术贝总数的比例)可评判育珠效果,与分析、综合评估结论一致,前者可量化评判数值,使评判更加直观,简易。
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| 关 键 词： | 马氏珠母贝 育珠 细胞小片 珠核 处理液 |
Treatment Techniques of Mantle Piece and Nucleus in Implantation of Pinctada martensii Ⅱ: The Experimentation of Pearl Producing with Different Treatment Solution in Xuwen, Guangdong |
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| WANG Mei-fang,YU Xiang-yong,LI Yong-mei,BIN CHENG-yong,DOU-wei,WAN Zheng-ping,CHEN Xiao-han.Treatment Techniques of Mantle Piece and Nucleus in Implantation of Pinctada martensii Ⅱ: The Experimentation of Pearl Producing with Different Treatment Solution in Xuwen, Guangdong[J].Journal of Zhanjiang Ocean University,2010,30(6). |
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| Authors: | WANG Mei-fang YU Xiang-yong LI Yong-mei BIN CHENG-yong DOU-wei WAN Zheng-ping CHEN Xiao-han |
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| Abstract: | Through the experiments of pearl culture in sea farm,further optimization and selection,treatment solution of mantle piece and pearl nuclear suitable for pearl culture sea area of Guangdong was discovered.The results show as the follows: 1) the matched appropriate treatment solution had an effect of reducing nucleus spitting rate.The nucleus spitting rate in Group W0,Y3 were only 31-32% which was obviously lower than those of the control group(46.2%).This suggested matched solution had an effect of reducing nucleus spitting rate significantly.2) The implanted oyster’s death,which has a connection with the behavior of nucleus spitting,occurs mainly at early stage of pearl culture,.3) The ingredient C in the treatment solution exerts influence on nucleus spitting rate.0.6% of C2 was not the suitable amount to be added in treatment solution.But adding 1% of C3 into treatment solution can reduce the rate of nucleus spitting.4) Programs suited for matching W0,W1,Y3 are selected.Namely,treatment solution C3 is the best match for W0,W1,Y3.W0 matched group,with 57.8% of survived oysters,67.5% of qualified pearls,is the most suitable group for pearl culture.5) After 8 months of culture,the number of produced pearl per 100 oysters was 101.2,and the rate of survival and pearls of high quality in group W0 were 57.8%,67.5% respectively,which increased significantly compared with traditional treatment group(control).6) Comparisons with different duration(8,10,13 months) of pearl culturing of Group W0,W1,Y3 were conducted.Y3 matched group has better effect on pearl culture when duration was as long as one year.7) The index of evaluation can be used to judge the quality of pearl culture.The evaluation results are the same with what have been analyzed and evaluated.But it is more intuitive to quantify the value judgment. |
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| Keywords: | Pinctada martensii pearl culture mantle piece pearl nuclei treatment solution |
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