| 三倍体牙鲆的核型证明 |
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| 引用本文： | 尤锋,刘静.三倍体牙鲆的核型证明[J].海洋与湖沼,1995,26(S1):115-118. |
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| 作者姓名： | 尤锋 刘静 |
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| 作者单位： | 中国科学院海洋研究所 青岛 266071;中国科学院海洋研究所 青岛 266071 |
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| 基金项目： | 山东省科委资助!983120－1 |
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| 摘 要： | 于1990，1993 - 1994年以捕自青岛近海的牙鲆亲鱼，采用冷休克法（0－4°C）进行多倍体的人工诱导实验，以期掌握牙鲆细胞遗传学特征和研究遗传育种方法。通过实验得到了三倍体牙鲆并进行培育。在原肠胚期采用空气干燥法、Giemsa染色获得正常二倍体、三倍体的染色体制片。镜检得，牙鲆二倍体染色体以2n=48，均为端部着丝点染色体，核型为24t，其管数NF=48；三倍体的染色体3n=72，也都为端部着丝点染色体，其臂数NF=72，并依据二倍体的枝型得出了三倍体的核型图。
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| 关 键 词： | 牙鲆 二倍体 三倍体 核型 |
| 收稿时间： | 1995/1/25 0:00:00 |
| 修稿时间： | 1995/4/17 0:00:00 |
KARYOTYPE EVIDENCES OF TRIPLO,IDY IN THE LEFT-EYED FLOUNDER, PARALICHTHYS OLIVACEUS (T. & S.) |
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| You Feng and Liu Jing.KARYOTYPE EVIDENCES OF TRIPLO,IDY IN THE LEFT-EYED FLOUNDER, PARALICHTHYS OLIVACEUS (T. & S.)[J].Oceanologia Et Limnologia Sinica,1995,26(S1):115-118. |
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| Authors: | You Feng and Liu Jing |
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| Institution: | Institute of Oceanology, Academia Sinica, Qingdao 266071;Institute of Oceanology, Academia Sinica, Qingdao 266071 |
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| Abstract: | Studies on triploidy induction in the left-eyed flounder, Paralichzhys alivaceur (T. & S.) from the coast of Qingdao were carried out using cold-shock technique (0 - 4°C) in 1990, 1993 and 1994 in Qingdao. The triploid left-eyed flounder were obtained and cultured under normal conditions. This paper presents karyotype evidences of induced triploidy and diploid left-eyed flounder. Samples for chromosome studies of diploidy and induced triploidy were prepared according to the routine air-dried method and stained with a Giemsa solution. Observation under microscope reveals that there are 48 chromosomes in diploidy which are acrocentric chromosomes and its karyotype formula is 24t, NF = 48, while in triploidy there are 72 chromosomes which are also acrocentric chromosomes and its arm number NF= 72.
The different chromosome numbers of diploidy in the left-eyed flounder obtained in this study were different from those of Nogosu (1960) and Sakamoto et al. (1980). We consider that there might be polymorphism in karyotypes of the left-eyed flounder due to the different geological populations. This study also showed that the early stage of triploidy in the left-eyed flounder could be identified very well in the gastrula stage and that its triploid karyotype is made on the basis of the diploid karyotype and the resources of chromosomes. |
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| Keywords: | Paralichthys olivaceus (T & S ) Diplody Triploidy Karyotype |
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