Triploidy induction in Pacific oysterCrassostrea gigas by caffeine with thermal shock |
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| Authors: | Zhang Guo-fan Chang Ya-qing Song Jian Ding Jun Shen Jin-kai Wang Yong-ping |
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| Institution: | (1) Key Laboratory of Maricultural Ecology, Ministry of Agriculture, PRC, Dalian Fisheries University, 116023 Dalian |
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| Abstract: | Zygotes of Pacific oyster (Crassostrea gigas) were treated for triploid induction with caffeine (10 mmol/L, 15mmol/L and 20mmol/L) in combination with thermal shocks
(at 40 minute post-fertilization) lasting for 5 and 10 minutes. The highest yield of triploids, 41.5%, was obtained from the
treatment with 20 mmol/L caffeine at 34°C lasting 10 minutes. The triploid levels were less than 30.0% in other treatments.
Triploid induction was more effective in treatment with 15–20 mmol/L caffeine at 34–38 °C than with lower concentrations of
caffeine at temperatures below 32 °C. Our results suggest that triploid induction with caffeine in combination with thermal
shocks is less efficient than some other methods reported previously. |
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| Keywords: | Crassostrea gigas triploid caffeine treatment thermal shock |
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