| 氮肥及秸秆有益于提高设施番茄土壤芽孢杆菌及假单胞菌的种群数量 |
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| 引用本文： | 葛晓颖,陈 清,孙志刚,何春娥,李涛,欧阳竹.氮肥及秸秆有益于提高设施番茄土壤芽孢杆菌及假单胞菌的种群数量[J].资源与生态学报（英文版）,2015,6(5):293-301. |
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| 作者姓名： | 葛晓颖 陈 清 孙志刚 何春娥 李涛 欧阳竹 |
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| 摘 要： |
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| 收稿时间： | 2015-10-02 |
Nitrogen and Straw Applications Increase Population Size of Bacillus and Pseudomonas spp. in Greenhouse Soil |
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| GE Xiaoying,CHEN Qing,SUN Zhigang,HE Chun-e,LI Tao,Ouyang Zhu.Nitrogen and Straw Applications Increase Population Size of Bacillus and Pseudomonas spp. in Greenhouse Soil[J].Journal of Resources and Ecology,2015,6(5):293-301. |
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| Authors: | GE Xiaoying CHEN Qing SUN Zhigang HE Chun-e LI Tao Ouyang Zhu |
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| Institution: | 1.Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, CAS, Beijing 100101, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China |
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| Abstract: | Root zone N management can reduce N fertilizer input and avoid nitrate pollution in greenhouse tomato production; however, little information is available on how reduced N affects soil microbes, especially dominant bacteria populations in long-term continuous greenhouse systems. This study investigated the effects of different combinations of N and straw on the population size of soil total bacteria and two dominant bacteria (Pseudomonas spp. and Bacillus spp.), and microbial community structure in a 6-year greenhouse tomato trial. The treatments were basal fertilizer (10 t ha-1 chicken manure; control), conventional high N treatment 600 kg N ha-1], conventional high N treatment with wheat straw, reduced N treatment 300 kg N ha-1 less than conventional], and reduced N treatment with wheat straw. Six years of reduced mineral N fertilization did not lead to a decrease in fruit yield. Both mineral N fertilizer and straw increased the numbers of soil bacteria (Bacillus spp. and Pseudomonas spp.) compared with the control, but the numbers were not affected by the amount of mineral N fertilizer (high vs reduced). Different denaturing gradient gel electrophoresis patterns were evidence that soil bacterial communities were changed by N treatments and straw application, but little difference in bacterial diversity was detected between high N and reduced N treatments. These results indicated that a reduction in N fertilizer input was possible in greenhouse tomato production and was beneficial in sustainable agriculture. |
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