Rapid,sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification |
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| Authors: | GAO Hongwei LI Fuhua ZHANG Xiaojun WANG Bing XIANG Jianhai Institute of Oceanology Chinese Academy of Sciences Qingdao China Sh ong Entry-Exit Inspection Quarantine Bureau of the People's Republic of China Qingdao China |
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| Institution: | GAO Hongwei,LI Fuhua,ZHANG Xiaojun,WANG Bing,XIANG Jianhai Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China Sh,ong Entry-Exit Inspection , Quarantine Bureau of the People's Republic of China,Qingdao266001,China |
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| Abstract: | Vibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming.The rapid detection and identification of V.anguillarum,and other pathogens that infect marine organisms,is crucial to effective disease management.In this study,we developed a loop-mediated amplification (LAMP) assay to detect V.anguillarum in an hour in a single tube without the need for thermal cycling.Conserved regions of the metalloproteinase (empA) gene of V.anguillarum served as the... |
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