Amylase production by the marine yeast Aureobasidium pullulans N13d |
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Authors: | Li Haifeng Chi Zhenming Wang Xiaohong and Ma Chunling |
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Institution: | (1) UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Qingdao, 266003, P. R. China |
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Abstract: | The marine yeast strain N13d, producing an extracellular amylase, was isolated from the deep sea sediments of the Pacific
Ocean. This strain was identified to be Aureobasidium pullulans by 18S rRNA gene sequence analysis and routine yeast identification methods. The optimal sea water medium for amylase production
by this yeast strain was 1.0% peptone and 1.0% soluble starch with pH 4.0. The optimal conditions for amylase production by
this yeast strain were with temperature 28 °C, aeration rate 6 Lmin−1 and agitation speed 250 rmin−1. Under these conditions, 58.5 units of amylase activity per mg protein were produced within 56 h of fermentation. |
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Keywords: | extracellular amylase marine yeast Aureobasidium pullulans fermentation |
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