The polypeptide in Chlamys farreri can protect human dermal fibroblasts from ultraviolet B damage |
| |
| Authors: | Zhang Yujiang Zhan Songmei Cao Pengli Liu Ning Chen Xuehong Wang Yuejun and Wang Chunbo |
| |
| Institution: | (1) Medical College, Qingdao University, 266021 Qingdao, Shandong, China;(2) Yellow Sea Fishery Research Institute, 266071 Qingdao, Shandong, China |
| |
| Abstract: | To investigate the effect of polypeptide fromChlamys farreri (PCF) on NHDFin vitro, we modeled oxidative damage on normal human dermal fibroblasts (NHDF) exposed to ultraviolet B (UVB). In this study, 3-4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium
bromide (MTT) and lactate dehydrogenase (LDH) were tested to measure cell viability. Enzymes including superoxide dismutase
(SOD), glutathione peroxidase (GSH-PX), catalase (CAT) and xanthine oxidase (XOD) were determined biochemically. Total antioxidative
capacity (T-AOC) and anti-superoxide anion capacity (A-SAC) were also determined. Ultrastructure of fibroblasts was observed
under transmission electron microscope. The results showed that: UVB (1.176×10−4 J/cm2) suppressed the growth of fibroblasts and the introduction of PCF (0.25%–1%) before UVB reduced the suppression in a concentration-dependent
manner. PCF could enhance the activities of SOD, GSH-PX and T-AOC as well as A-SAC. Also PCF could inhibit XOD activity, while
it did not affect CAT activity. Ultrastructure of fibroblasts were damaged after UVB irradiation, concentration-dependent
PCF reduced the destructive effect of UVB on cells. These results indicated that PCF can protect human dermal fibroblasts
from being harmed by UVB irradiation via its antioxidant proerty.
This work was supported by the National Natural Science Foundation of China (No. 39970638) and the Science and Technology
Bureau of Qingdao (No. 2001-28-50). |
| |
| Keywords: | polypeptide fromChlamys farreri ultraviolet ray oxygen free radicals antioxidant human dermal fibroblast |
| 本文献已被 SpringerLink 等数据库收录！ |
|
