| 一株海水氨氧化细菌的系统发育分析 |
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| 引用本文： | 黄建军,简纪常,吴灶和,丁燏.一株海水氨氧化细菌的系统发育分析[J].广东海洋大学学报,2008,28(3). |
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| 作者姓名： | 黄建军 简纪常 吴灶和 丁燏 |
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| 作者单位： | 广东海洋大学水产学院,广东省水产经济动物病原生物学及流行病学重点实验室暨广东省教育厅水产经济动物病害控制重点实验室,广东,湛江,524025 |
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| 摘 要： | 应用PCR技术扩增16S rRNA基因和amoA(ammonia monooxygenase subunit A)的基因片段,并测定其序列,对一株源于海水养殖水体的高效氨氧化细菌(ammonia-oxidizing bacteria,AOB)进行了系统发育分析。结果表明,经PCR扩增得到了1 098 bp的16S rRNA基因片段和491 bp的amoA基因片段,将其序列用NCBI-Blast软件在GenBank数据库中进行同源性检索后发现,该菌株的16S rRNA基因序列和amoA基因序列分别与亚硝化单胞菌Nitrosomonas sp.NS20的相对应基因片段相似性分别为98.4%和96.7%。在此基础上构建了系统发育树,表明用该菌株的16S rRNA基因片段和amoA基因片段构建的系统发育树均与亚硝化单胞菌属类聚在一起,结合该菌株形态和生理生化特性,鉴定该株氨氧化细菌属亚硝化单胞菌。
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| 关 键 词： | 氨氧化细菌 16SrRNA amoA 聚合酶链反应 同源性分析 |
Phylogenetic Analysis of a Strain of Marine Ammonia-Oxidizing Bacterium |
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| HUANG Jian-jun,JIAN Ji-chang,WU Zao-he,DING Yu.Phylogenetic Analysis of a Strain of Marine Ammonia-Oxidizing Bacterium[J].Journal of Zhanjiang Ocean University,2008,28(3). |
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| Authors: | HUANG Jian-jun JIAN Ji-chang WU Zao-he DING Yu |
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| Abstract: | A strain of marine ammonia-oxidizing bacterium(AOB),N2005 isolated from marine aquaculture water was phylogenetic analyzed.Total DNA of the bacteria was extracted,and the fragments of the 16S rRNA gene and ammonia monooxygenase subunit A(amoA) gene were amplified by polymerase chain reaction(PCR).The amplified fragments of 16S rRNA gene and amoA gene were 1098 bp and 491 bp,respectively.Homology was analyzed by searching in the GenBank with NCBI-Blast software.The results showed that the amplified fragments had high homology with the 16S rRNA gene and amoA gene of Nitrosomonas sp.NS20(98% and 96%,respectively).Based on the 16S rRNA and amoA sequence,phylogenetic tree of the system was constructed,showing that the ammonia-oxidizing bacterium in present study should belong to Nitrosomonas. |
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| Keywords: | ammonia-oxidizing bacteria 16S rRNA amoA polymerase chain reaction homology analysis |
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