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| 用FISH技术检测太湖梅梁湾水体中细菌优势种群在冬夏两季的变化 | |
| 引用本文： | 沈燕,高光,汤祥明,晁建颖.用FISH技术检测太湖梅梁湾水体中细菌优势种群在冬夏两季的变化[J].湖泊科学,2010,22(1):57-62. |
| 作者姓名： | 沈燕 高光 汤祥明 晁建颖 |
| 作者单位： | 1. 中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室,南京,210008;南京农业大学,南京,210095 2. 中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室,南京,210008 3. 中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室,南京,210008;中国科学院研究生院,北京,100049 |
| 基金项目： | 中国科学院知识创新工程重要方向性项目(KZCX2-YW-419);;国家重点基础研究发展计划项目(2008CB418103);;国家自然科学基金项目(40730529)联合资助 |
| 摘 要： | 采用FISH技术对2008年冬、夏两季太湖梅梁湾水体中细菌优势种群的丰度进行了对比分析。实验中,选取荧光素标记的特异寡核苷酸探针,与太湖梅梁湾水体中的四类细菌优势种群α-变形菌(α-proteobacteria),β-变形菌(β-proteobacteria),γ-变形菌(γ-proteobacteria)和拟杆菌(Bacteroidetes)进行原位杂交。结果表明:①所测定的四类细菌优势种群占总细菌的比例在冬季均显著高于夏季(P0.01);②水体中α-proteobacteria的丰度随TP浓度的增加而显著增加(R~2=0.860,P0.01);③随着Chl.a浓度的增加,水体中细菌的丰度呈上升的趋势,尤其是其中的γ-proteobacteria,其增大趋势更加显著(R~2=0.857,P0.01);④细菌种群的丰度受水体中PO_4~(3-)浓度的影响,尤其是对Bacteroidetes的影响更加显著(R~2=0.733,P0.05)。 |
| 关 键 词： | 太湖 荧光原位杂交 优势细菌 丰度 |
| 收稿时间： | 2009/5/10 0:00:00 |
| 修稿时间： | 2009/5/21 0:00:00 |
Variation of bacterial dominated population abundance in Meiliang Bay, Lake Taihu detected by FISH during summer and winter |
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| SHEN Yan,Gao Guang,Tang Xiangming and Zhao Jianying.Variation of bacterial dominated population abundance in Meiliang Bay, Lake Taihu detected by FISH during summer and winter[J].Journal of Lake Science,2010,22(1):57-62. | |
| Authors: | SHEN Yan Gao Guang Tang Xiangming and Zhao Jianying |
| Institution: | 1;3;1;2;1;2;1:State Key Laboratory of Lake Science and Environment;Nanjing Institute of Geography and Limnology;Chinese Academy of Sciences;Nanjing 210008;P.R.China;2:Graduate School of Chinese Academy of Sciences;Beijing 100049;P.R.China;3:Nanjing Agricultural University;Nanjing 210095;P.R.China |
| Abstract: | FISH was used to analyze the abundance of dominated bacteria population in Meiliang Bay of Lake Taihu in winter and summer in 2008.The four dominated bacteria population(α-proteobacteria,β-proteobacteria,γ-proteobacteria,Bacteroidetes)were analyzed by in situ hybridization with specific fluorescently(FITC)labeled rRNA-targeted oligonucleotide probes in the test.The results indicated:①The proportion of each of the four dominated species in the total number of bacteria was all significantly higher in winter than that in summer(P ＜ 0.01);②the abundance of α-proteobacteria rose notably with the increase of TP concentration (R~2 =0.860,P ＜0.01);③ there was ascending trend in the abundance of bacteria along with the increase of Chl.a concentration,especially that of γ-proteobacteria was more remarkable than the others(R~2 =0.857,P ＜0.01);④ the abundance of bacteria population,especially for Bacteroidetes,was also influenced by PO_4~(3-) concentration(R~2=0.733,P ＜0.05). |
| Keywords: | Lake Taihu FISH dominated bacterial abundance |
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