Effect of nitrate and phosphate on accumulation of β-carotene isomers inDunaliella salina |
| |
| Authors: | Liu Jian-guo Wu Chao-yuan Chen Nian-hong Wang Yu-jun Yu Li-dong |
| |
| Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao |
| |
| Abstract: | Dunaliella salina, a halotolerant unicellular green alga, can accumulate a large amount of β-carotene under certain environmental conditions.
The isomers of β-carotene extracted fromD. salina cultured in medium with different nitrate and phosphate concentrations were analysed by HPLC with Alox-T alumina column.
At least six isomers were found in different proportions depending on the culture media's nitrate and/or phosphate concentrations.
Nitrate and/or phosphate deficiency was conducive to the accumulation of total cis isomers but not of all trans isomer. It
is suggested that 1 mmol/L KNO3 and 0.1 mmol/L KH2 PO4 are favourable for accumulation of total cis β-carotene.
Contribution No. 2090 from the Institute of Oceanology, Chinese Academy of Sciences.
This project was supported by the National Natural Science Foundation of China, Grant No. 38970587. |
| |
| Keywords: | Dunaliella salina β -carotene isomer cis and trans isomers accumulation nitrate and phosphate effect |
| 本文献已被 SpringerLink 等数据库收录！ |
