| Preliminary Validation of High Performance Liquid Chromatography Method for Detection of Methyltestosterone Residue in Carp Muscle |
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| 作者姓名： | JIANG Jie LIN Hong FU Xiaoting LI Mingming |
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| 作者单位： | College of Food Science and Engineering, Ocean University of China, Qingdao 266003, P.R. China |
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| 摘 要： | The use of synthetic anabolic steroid methyltestosterone （MT） as growth promoter is prohibited in China. Validations of analytical methods for MT residue in food and the results obtained have become indispensable. The high performance liquid chromatography （HPLC） method for the detection of MT with liquid-liquid extraction by trichloromethanemethanol in carp muscle tissue was preliminarily validated with reference to the following parameters： recovery （accuracy） at the 1, 5 and 10 mgkg^-1 level, between-run and within-run CV values （repeatability, also called relative standard deviation （RSD）） and limit of detection. The recoveries were above 80% and the between-run and within-run CV values below 10% for muscle tissue. The limit of detection was 0.05 mgkg^-1.
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| 关 键 词： | 鲤鱼 睾丸激素 HPLC 液体萃取法 |
| 文章编号： | 1672-5182（2005）03-248-04 |
| 收稿时间： | 2005-04-04 |
| 修稿时间： | 2005-06-13 |
Preliminary validation of high performance liquid chromatography method for detection of methyl-testosterone residue in carp muscle |
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| JIANG Jie LIN Hong FU Xiaoting LI Mingming.Preliminary Validation of High Performance Liquid Chromatography Method for Detection of Methyltestosterone Residue in Carp Muscle[J].Journal of Ocean University of China,2005,4(3):248-251. |
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| Authors: | Jie Jiang Hong Lin Xiaoting Fu Mingming Li |
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| Institution: | College of Food Science and Engineering, Ocean University of China, Qingdao 266003, P.R. China |
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| Abstract: | The use of synthetic anabolic steroid methyltestosterone (MT) as growth promoter is prohibited in China. Vali-dations of analytical methods for MT residue in food and the results obtained have become indispensable. The high perfor-mance liquid chromatography (HPLC) method for the detection of MT with liquid-liquid extraction by trichloromethane-methanol in carp muscle tissue was preliminarily validated with reference to the following parameters: recovery (accuracy)at the 1, 5 and 10mg kg-1 level, between-run and within-run CV values (repeatability, also called relative standard deviation(RSD)) and limit of detection. The recoveries were above 80% and the between-run and within-run CV values below 10%for muscle tissue. The limit of detection was 0.05 mg kg-1. |
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| Keywords: | methyltestosterone liquid-liquid extraction HPLC validation |
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