Natural clays usually show anisotropic stiffness due to their deposition process and anisotropic in situ stress state. The stiffness anisotropy depends on both of the stress anisotropy and fabric anisotropy, while the latter can be quantified by the stiffness anisotropy at isotropic stress states. This paper measures the K₀ value (i.e., stress anisotropy) and elastic shear stiffness anisotropy of natural Shanghai clay in a triaxial apparatus with horizontal and vertical bender elements. The results show that the K₀ value of Shanghai clay lies in the range of 0.40–0.66, and an empirical equation is proposed to estimate the K₀ value based on the plasticity index and initial void ratio. The fabric anisotropy of natural Shanghai clay lies in the range of 1.2–1.4 with a stronger fabric in the horizontal plane. Moreover, the experimental data of the stiffness anisotropy and fabric anisotropy of different clays in the literature are reviewed and analyzed. It reveals that the stiffness anisotropy generally increases, while the fabric anisotropy remains nearly the same during K₀ consolidation. For normally consolidated clay, the fabric anisotropy generally lies in the range of 1.1–1.7. For overconsolidated clays, the fabric anisotropy generally increases as the overconsolidation ratio increases. Empirical equations are proposed to approximately estimate the fabric anisotropy of clays based on its stress normalized elastic shear stiffness.