Antioxidant efficacy of extracts from a variety of seaweeds in a cellular system |
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| Authors: | You Ah Kim Chang Suk Kong Young Ran Um Jung Im Lee Taek Jeong Nam Youngwan Seo |
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| Institution: | (1) Division of Marine Environment & Bioscience, Korea Maritime University, 606-791 Busan, Korea;(2) Research Institute of Marine Science and Technology, Korea Maritime University, 606-791 Busan, Korea;(3) Faculty of Food Science and Biotechnology, Pukyong National University, 608-737 Busan, Korea |
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| Abstract: | As a part of an ongoing search for antioxidants frodm marine sources, antioxidant activities of 24 kinds of seaweeds (4 green
algae, 8 brown algae, and 12 red algae) were investigated. The seaweeds were extracted by acetone/ dichloromethane and methanol,
respectively. The antioxidant properties of both extracts were evaluated using four different activity tests, including degree
of occurrence of intracellular reactive oxygen species (ROS), NO, lipid peroxidation, and GSH (glutathione) in mouse macrophage
Raw 264.7 cells. The levels of intracellular reactive oxygen species (ROS) and GSH were measured using 2’,7’-dichlorofluorescin
diacetate (DCF-DA) and monobromobimane as fluorescence probe, respectively. Moreover, the generation of NO and lipid peroxidation
products were determined by each method based on the Griess reaction and TBARS assay. Solvent extracts from seaweeds such
asScytosiphon lomentaria, Prionitis cornea, Laruencia okamurae,Callophyllis japonica, Sargassum horneri, Dictyopteris divaricat a,Lomentaria catenata, Corallina confuse, Ishige okamurae, andAhnfeltiopsis flabelliformi exhibited high antioxidant activities in cellular oxidizing systems. |
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| Keywords: | seaweeds reactive oxygen species nitric oxide lipid peroxidation glutathione |
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