In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica |
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| Authors: | Jing Wang Quanbin Zhang Zhongshan Zhang Yun Hou Hong Zhang |
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| Institution: | (1) Department of Marine Biotechnology, Cheju National University, 66 Jejudaehakno, Jeju, 690-756, Republic of Korea;(2) Department of Veterinary Medicine, Cheju National University, 66 Jejudaehakno, Jeju, 690-756, Republic of Korea; |
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| Abstract: | Fucoidan, a group of sulfated heteropolysaccharides, was extracted from Laminaria japonica, an important economic alga species in China. The anticoagulant activity of fucoidan and its derivatives (including sulfated,
phosphorylated, and aminated fucoidan) was examined using in-vitro anticoagulant systems. The correlation between chemical
variations within the fucoidan group and anticoagulant activity was determined. The in-vitro anticoagulant properties of fucoidan
and its derivatives were determined by measuring activated partial thromboplastin time (APTT), prothrombin time (PT), and
thrombin time (TT). The results indicate anticoagulant activity in all samples using APTT and TT assays; however, only the
fucoidan derivatives affected the PT assay. Thus, the fucoidan derivatives were able to inhibit both intrinsic and extrinsic
blood coagulants. Fucoidan (FPS) and its derivatives presented better anticoagulant activity than low molecular weight fucoidan
(DFPS) and its derivatives, suggesting that molecular weight and proper conformation are contributing factors for anticoagulant
activity of polysaccharides. Amino groups have a positive charge and can thus change the charge density of fucoidan. Accordingly,
among the tested samples, aminated fucoidan (NF) was the most active reflecting the importance of charge density for anticoagulant
activity. Available data obtained using in-vitro models suggest that the sulfate content, sulfate/total-sugar ratio, molecular
weight, and the substituted group of fucoidan are important factors for anticoagulant activity but that the influence of sulfate,
phosphate and amino groups on anticoagulant activity was different. |
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