| Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences |
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| 作者姓名： | WANG Jun ZHANG Wen SU Yongquan DING Shaoxiong |
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| 作者单位： | CollegeofOceanographyandEnvironmentalScience,XiamenUniversity,Xiamen361005,China |
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| 基金项目： | 国家高技术研究发展计划(863计划)，福建省科研项目 |
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| 摘 要： | The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.
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| 关 键 词： | 28S rRNA 序列分析 基因研究 单殖亚纲动物 聚合酶链 |
| 收稿时间： | 2003/10/11 0:00:00 |
| 修稿时间： | 2004/1/20 0:00:00 |
Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences |
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| WANG Jun,ZHANG Wen,SU Yongquan,DING Shaoxiong.Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences[J].Acta Oceanologica Sinica,2004,23(4):709-716. |
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| Authors: | WANG Jun ZHANG Wen SU Yongquan and DING Shaoxiong |
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| Institution: | College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China |
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| Abstract: | The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellaeand N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers andthen sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on thedifferent fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher thanthat of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% to11.73%. In addition, UPGMA and MP molecularphylogenetic trees are constructed and proved to be consistent with each other. Though the morphologicalcharacteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether theybelong to a single species or not are still undefined, and the more genes of them should be further investigated, incombination with the systematical and detailed morphological study. |
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| Keywords: | S rRNA Neobenedenia sequence analysis |
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