Detection of nitrogenase in individual cells of a natural population of <Emphasis Type="Italic">trichodesmium</Emphasis> using immunocytochemical methods for fluorescent cells |
| |
| Authors: | Kaori Ohki Yukiko Taniuchi |
| |
| Institution: | (1) Department of Marine Bioscience, Faculty of Marine Bioscience, Fukui Prefectural University, Gakuencho, Obama, Fukui 917-0003, Japan;(2) Present address: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaoshiung, 80424, Taiwan |
| |
| Abstract: | The intercellular distribution of nitrogenase was detected in a natural population of Trichodesmium spp. collected from Shitaba Bay, Uwajima, Ehime Prefecture, Japan. Polyclonal antibodies raised against the Fe-protein and
the MoFe-protein (α-subunit) of nitrogenase were used as probes. Visualization of the antibody binding to the nitrogenase
was performed using horseradish peroxidase-conjugated secondary antibody and the chromogenic substrate, 3,3′-diaminobenzidine
tetrahydrochloride, to avoid the masking effects caused by the bright autofluorescence emitted by the Trichodesmium cells. Nitrogenase proteins were detected in 66 to 81% of the trichomes. More than 77% of the cells were immunostained in
individual nitrogenase-containing trichome. The data show natural populations of Trichodesmium spp. do not developed heterocyst-equivalent cells. |
| |
| Keywords: | Natural population of Trichodesmium spp nitrogenase synthesis whole-cell immunolocalization diazotrophic cyanobacteria |
| 本文献已被 SpringerLink 等数据库收录！ |
|
