| 南海有毒塔玛亚历山大藻的分子地理标记分析 |
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| 引用本文： | 陈月琴,邱小忠,屈良鹄,曾陇梅,齐雨藻,郑磊.南海有毒塔玛亚历山大藻的分子地理标记分析[J].海洋与湖沼,1999,30(1):45-51. |
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| 作者姓名： | 陈月琴 邱小忠 屈良鹄 曾陇梅 齐雨藻 郑磊 |
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| 作者单位： | 中山大学生命科学学院，中山大学生命科学学院，中山大学生命科学学院，中山大学生命科学学院化学化工学院，暨南大学水生生物研究所，暨南大学水生生物研究所 广州，510275，广州，510275，广州，510275，广州，510275，广州，510632，广州，510632 |
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| 基金项目： | “九五”国家自然科学基金!39790110 |
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| 摘 要： | 采用聚合酶链扩增反应（ＰＣＲ）和限制性片段长度多态性分析（ＲＦＬＰ）方法，对１９９２年５月和１９９４年１２月采自中国南海大鹏湾，大亚湾，香港海域的赤潮有毒甲藻塔玛亚历山大藻８个不同地理株的核糖体小亚基ＲＮＡ基因（Ｓｓ－ｒＤＮＡ）进行分析，并与北美，西欧等地的比较。结果表明，中国南海的塔玛业历山大藻缺少Ｂ基因，这与北美等地的塔玛亚历山大藻（包括有毒和无毒株）不同，而与西欧，澳洲等地的有毒和无毒株相似
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| 关 键 词： | 塔玛亚历山大藻 分子地理标记 南海 有毒藻 红潮 |
| 收稿时间： | 1997/3/18 0:00:00 |
| 修稿时间： | 1998/8/28 0:00:00 |
ANALYSIS OF MOLECULAR BIOGEOGRAPHIC MARKER ON RED TIDE TOXIC ALEXANDRIUM TAMARENSE IN THE SOUTY CHINA SEA |
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| CHEN Yue-qin,QIU Xiao-zhong,QU Liang-hu,ZENG Long-mei,QI Yu-zao and ZHENG Lei.ANALYSIS OF MOLECULAR BIOGEOGRAPHIC MARKER ON RED TIDE TOXIC ALEXANDRIUM TAMARENSE IN THE SOUTY CHINA SEA[J].Oceanologia Et Limnologia Sinica,1999,30(1):45-51. |
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| Authors: | CHEN Yue-qin QIU Xiao-zhong QU Liang-hu ZENG Long-mei QI Yu-zao and ZHENG Lei |
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| Institution: | School of Life Sciences,School of Chemistry and Chemical Technology, Zhongshan University,Guangzhou,510275;School of Life Sciences,School of Chemistry and Chemical Technology, Zhongshan University,Guangzhou,510275;School of Life Sciences,School of Chemistry and Chemical Technology, Zhongshan University,Guangzhou,510275;School of Chemistry and Chemical Technology, Zhongshan University,Guangzhou,510275;Institute of Hydrobiology,Jinan University,guanzhou,510632;Institute of Hydrobiology,Jinan University,guanzhou,510632 |
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| Abstract: | In this study, cultures of eight strains of red the toxic Alexandrium tamarense wereestablished from germinated cysts or vegetative cells isolated from Dapeng Bay, Deya Bay and theHong Kong area in May l992 and December l994, and a method of PCR amplification withindividual cel1s was used to amplify the small subtrit ribosomal RNA genes (Ss-00) of eightstrains for a restriction fragment length Polymorphism (RFLP) assay. PCRRFLP analysis of Ss-rDNAon the red the toxic A lamarense demonstlated that the strains of A lamarense in the South ChinaSea have Ss-rDNA A gene and lack of B gene, which was the same with that of the strains inwestem Japan and Austl-alia (toxic or nontoxic), but different from that of the strains in NorthAlnerican seas. The genetic consistence of toxic A tamarense in China seas suggested that theincrease in the geographic range of A tamarense in ths sea area has Possibly a local origin. Inaddiholl, it was demonstrated that the type of Ss-rDNA could be as a report marker for theidentification and monitoring of the origin and dispersal of toxic species of Alexandrium. |
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| Keywords: | Alexandrium tamarense Molecular geographic marker Small subunit ribosomal RNA genes (Ss-rDNA) RFLP Red tide |
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