| 泥蚶HDAC1基因cDNA全长、内含子克隆及时空表达特征分析 |
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| 引用本文： | 任付真,姚韩韩,董迎辉,周小龙,林志华.泥蚶HDAC1基因cDNA全长、内含子克隆及时空表达特征分析[J].海洋学报,2016,38(8):73-82. |
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| 作者姓名： | 任付真 姚韩韩 董迎辉 周小龙 林志华 |
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| 作者单位： | 1.上海海洋大学 水产与生命学院, 上海 201306;浙江万里学院 生物与环境学院 浙江省水产种质资源高效利用技术研究重点实验室, 浙江 宁波 315100 |
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| 基金项目： | 国家现代贝类产业技术体系项目（CARS-48）；浙江省自然科学基金重点项目（LZ12C19001）；浙江省重大科技专项（2012C12907-4）；国家水产种质资源平台项目（2015DKA30470）。 |
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| 摘 要： | HDAC1作为HDACs家族中重要成员，可使组蛋白去乙酰化进而调节基因表达，在细胞分化和胚胎早期发育中起重要作用。本文利用SMART RACE技术克隆得到泥蚶HDAC1(Tg-HDAC1)基因的cDNA全长序列，并对其内含子进行扩增及不同组织、不同发育时期的定量表达。结果发现:Tg-HDAC1基因的cDNA序列全长为2275 bp，开放阅读框(ORF)1587 bp，编码528个氨基酸；Tg-HDAC1蛋白序列与斑马鱼、鸡、小鼠等的相似性都在80%以上，表明该蛋白氨基酸序列在物种进化过程中具有保守性；在Tg-HDAC1基因中扩增出13个内含子，均存在于开放阅读框中，且都遵循GT-AG原则；荧光定量PCR(qRT-PCR)分析结果显示，Tg-HDAC1基因在成体血液、内脏团、外套膜、鳃、斧足和闭壳肌6个组织中均有表达，而在足中的表达量最高，且与其余5组织中的表达量有极显著差异(P<0.01)，说明它对该组织生长具有重要作用。不同发育时期的表达差异结果表明，Tg-HDAC1基因在担轮幼虫期表达量最高，显著高于其他发育时期(P<0.05)。
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| 关 键 词： | 泥蚶 组蛋白去乙酰化酶1(HDAC1) 基因克隆 内含子 表达分析 |
| 收稿时间： | 2015/10/23 0:00:00 |
| 修稿时间： | 4/6/2016 12:00:00 AM |
cDNA, introns cloning and spatiotemporal expression analysis of HDAC1 gene in Tegillarca granosa |
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| Ren Fuzhen,Yao Hanhan,Dong Yinghui,Zhou Xiaolong and Lin Zhihua.cDNA, introns cloning and spatiotemporal expression analysis of HDAC1 gene in Tegillarca granosa[J].Acta Oceanologica Sinica (in Chinese),2016,38(8):73-82. |
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| Authors: | Ren Fuzhen Yao Hanhan Dong Yinghui Zhou Xiaolong and Lin Zhihua |
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| Institution: | 1.College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China2.Key Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China3.College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | As an important member of HDACs family, HDAC1 can regulate gene expresstion and play a crucial role in cell differentiation and early embryonic development. Tg-HDAC1 cDNA was cloned by SMART RACE technique and then the bioinformatics, expression analysis, and intron amplification of Tg-HDAC1 were carried out in Tegillarca granosa. The full length of Tg-HDAC1 cDNA was 2 275 bp, containing a complete 1587 bp ORF encoding 528 amino acids. The homologous similarity between the blood clam and other species, such as Danio rerio, Gallus gallus, Mus musculus, was more than 80%, which indicate that HDAC1 is relatively conserved in the evolution. Thirteen introns of Tg-HDAC1 were amplified in ORF, which all of them follow the principle of GT-AG. The results of six tissue-specific expression by real time PCR showed that Tg-HDAC1 gene expressed in all tissues, and the expression of foot were significantly higher than other tissues(P<0.01), which suggest that the gene play an important role in the course of foot growth. The relative expression in different stages revealed that the expression of Tg-HDAC1 gradually increased with the process of the development, and showed the highest in trochophore stage (P<0.05). |
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| Keywords: | Tegillarca granosa HDAC1 gene cloning intron expression analysis |
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