Analysis of new microsatellite markers developed from reported sequences of Japanese flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis> |
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| Authors: | Haiyang Yu Liming Jiang Wei Chen Xubo Wang Zhigang Wang Quanqi Zhang |
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| Institution: | College of Marine Life Sciences,Key Laboratory of Marine Genetics and Breeding,Ministry of Education,Ocean University of China,Qingdao 266003,P.R.China |
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| Abstract: | The expressed sequence tags (ESTs) of Japanese flounder, Paralichthys olivaceus, were selected from GenBank to identify simple sequence repeats (SSRs) or microsatellites. A bioinformatic analysis of 11111
ESTs identified 751 SSR-containing ESTs, including 440 dinucleotide, 254 trinucleotide, 53 tetranucleotide, 95 pentanucleotide
and 40 hexanucleotide microsatellites respectively. The CA/TG and GA/TC repeats were the most abundant microsatellites. AT-rich
types were predominant among trinucleotide and tetranucleotide microsatellites. PCR primers were designed to amplify 10 identified
microsatellites loci. The PCR results from eight pairs of primers showed polymorphisms in wild populations. In 30 wild individuals,
the mean observed and expected heterozygosities of these 8 polymorphic SSRs were 0.71 and 0.83 respectively and the average
PIC value was 0.8. These microsatellite markers should prove to be a useful addition to the microsatellite markers that are
now available for this species. |
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| Keywords: | bioinformatics data mining microsatellite Japanese flounder EST |
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