Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (<Emphasis Type="Italic">R</Emphasis>)-methyl mandelate |
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| Authors: | Jiayuan Liang Aijun Sun Yun Zhang Dun Deng Yongfei Wang Sanmei Ma Yunfeng Hu |
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| Institution: | 1.Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou,China;2.Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou,China;3.South China Sea Bio-Resource Exploitation and Utilization,Collaborative Innovation Center,Guangzhou,China;4.Department of Biotechnology,Jinan University,Guangzhou,China |
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| Abstract: | Genomic mining has identified a novel microbial alkaline esterase from the Indian Ocean. This esterase was overexpressed in E. coli BL21 (DE3) and further functionally characterized. Under optimal conditions (10 mmol/L substrate, pH 6.0, 2 h at 40 °C), this esterase can hydrolyze racemic methyl mandelate to (R)-methyl mandelate with very high optical purity (e.e. >99%) and yield (nearly 90%). Interestingly, the stereoselectivity of this esterase is opposite to that of two previously reported lipases that can generate (S)-methyl mandelate through the hydrolysis of racemic methyl mandelate. No organic solvents or other additives were required to optimize the optical purity and production of the final chiral product (R)-methyl mandelate, which can potentially simplify the production procedure of (R)-methyl mandelate catalyzed by esterase. |
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