Activation of macrophages by an exopolysaccharide isolated from Antarctic <Emphasis Type="Italic">Psychrobacter</Emphasis> sp. B-3 |
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| Authors: | Leiye Yu Guojie Sun Jingfang Wei Yingze Wang Chao Du Jiang Li |
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| Institution: | 1.College of Bioscience and Bioengineering,Hebei University of Science and Technology,Hebei,China;2.Key Laboratory of Marine Bioactive Substances, First Institute of Oceanography,State Oceanic Administration (SOA),Qingdao,China |
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| Abstract: | An exopolysaccharide (EPS) was isolated and purified from an Antarctic psychrophilic bacterium B-3, identified as Psychrobacter sp., and the activation of RAW264.7 cells by B-3 EPS was investigated. The results show that B-3 EPS, over a certain concentration range, promoted cell viability, nitric oxide production, tumor necrosis factor (TNF)α secretion, and phagocytic ability. Furthermore, TAK-242, an inhibitor of the toll-like receptor 4 (TLR4) significantly reduced nitric oxide production by these cells after stimulation with B-3 EPS. Moreover, B-3 EPS induced p65 phosphorylation and IκBα degradation in these cells. In conclusion, B-3 EPS might have activated RAW264.7 cells by combining with TLR4 on cell surface and triggering activation of NF-κB signaling pathways, implying that this EPS could activate macrophages and regulate initial immune response. |
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