| 复方中草药对珍珠龙胆石斑鱼生长、非特异性免疫及消化酶活性的影响 |
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| 引用本文： | 徐安乐,黎中宝,上官静波,胡晓伟,黄永春,林茂松,林泽滨.复方中草药对珍珠龙胆石斑鱼生长、非特异性免疫及消化酶活性的影响[J].海洋学报,2018,40(12):49-57. |
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| 作者姓名： | 徐安乐 黎中宝 上官静波 胡晓伟 黄永春 林茂松 林泽滨 |
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| 作者单位： | 1.集美大学 水产学院, 福建 厦门 361021;福建省海洋渔业资源与生态环境重点实验室, 福建 厦门 361021 |
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| 基金项目： | 厦门市科技项目（3502Z20153009）；厦门市海洋经济发展专项资金项目（14CZY045HJ19）。 |
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| 摘 要： | 中草药含有多种活性物质，具有抗病助长无残留等特性，在国内外被广泛使用。本试验采用单因素试验设计方法，研究复方中草药（由黄芪、党参、大黄等12种中草药组合而成）不同剂量对珍珠龙胆石斑鱼（Epinephelus lanceolatus ♂×Epinephelus fuscoguttatus♀）生长性能、非特异性免疫及消化酶活性的影响。以初始体质量（19.16±0.25） g，体长为（10.63±0.56） cm的珍珠龙胆石斑鱼为试验对象，在基础饲料中分别添加0、0.4%、0.8%、1.2%、1.6%和2%复方中草药连续饲喂珍珠龙胆28 d。结果显示，该种复方中草药在添加剂量1.6%以内时，能有效降低石斑鱼的饵料系数（P<0.05），对石斑鱼的增重率和特定生长率影响不显著（P>0.05），但能显著提高石斑鱼血清中总蛋白含量（TP）、碱性磷酸酶（AKP）、酸性磷酸酶（ACP）以及总超氧化物歧化酶（T-SOD）活性（P<0.05）。对血清中非特异性免疫酶活性影响结果表明，中草药添加剂量在0.8%和1.2%时能很好地提高石斑鱼的免疫能力。石斑鱼肠道中的脂肪酶、胰蛋白酶活性受该种复方中草药的影响显著（P<0.05），在添加量为1.2%以内显著提高了肠道中脂肪酶活性，胰蛋白酶活性和肠道总蛋白含量，而对淀粉酶活性影响不明显（P>0.05），中草药对珍珠龙胆石斑鱼肠道消化酶活性的影响在添加剂量为1.2%时尤为明显。综合石斑鱼的生长、非特异性免疫和消化性能结果，认为该种复方中草药的最适添加量为1.2%左右。
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| 关 键 词： | 珍珠龙胆石斑鱼 中草药 生长性能 消化酶 非特异性免疫 |
| 收稿时间： | 2018/1/5 0:00:00 |
| 修稿时间： | 2018/2/11 0:00:00 |
Effects of compound Chinese herbal medicine on growth performance,non-specific immunity and digestive enzyme activity of Pearl gentian grouper |
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| Xu Anle,Li Zhongbao,Shangguan Jingbo,Hu Xiaowei,Huang Yongchun,Lin Maosong and Lin Zebin.Effects of compound Chinese herbal medicine on growth performance,non-specific immunity and digestive enzyme activity of Pearl gentian grouper[J].Acta Oceanologica Sinica (in Chinese),2018,40(12):49-57. |
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| Authors: | Xu Anle Li Zhongbao Shangguan Jingbo Hu Xiaowei Huang Yongchun Lin Maosong and Lin Zebin |
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| Institution: | 1.Fisheries College, Jimei University, Xiamen 361021, China;Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen 361021, China2.Dongshan Xiangyuanhui Aquaculture Company, Fujian Province, Dongshan 363400, China |
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| Abstract: | Chinese herbal medicine contains various active substances, with no residual fuel resistant characteristics, is widely used in China and abroad. To evaluate the effect of compound Chinese herbal medicine which consists of Astragalus, Codonopsis, Thubarb and other 9 kinds of Chinese herb medicine on growth performance, serum non-specific immunity and digestive enzyme activities of Pearl gentian grouper, 6 diets adding 0%, 0.4%, 0.8%, 1.2%, 1.6%, 2.0% compound Chinese herbal medicine were respectively fed to Pearl gentian grouper (19.16±0.25) g for 28 days. The results showed that with in the amount of 1.6%, Pearl gentian grouper feed conversion ratio significantly reduced (P<0.05), while the weight gain ratio and the specific growth ratio in each group showed no significant with the control group (P>0.05). The compound Chinese herbal medicine can enhance the content of total protein and the activities of Alkaline phosphatase (AKP), acid phosphatase (ACP) and total Superoxide dismutase (T-SOD)in serum significantly (P<0.05). Based on the effect of the results in the serum non specific immune enzyme activity, it proves that during an amount of addition 0.8% and 1.2% can well improve the immunity of Pearl gentian grouper. The activity of amylase, lipase, total protein content and trypsin were significantly influenced (P<0.05), but no apparent effect on the activity of amylase (P>0.05). Seen from the effect of compound Chinese herbal medicine on digestion enzyme in the gut, it suggests that the best adding level is 1.2%. Comprehensive grouper growth, digestion and non specific immunity results showed that this kind of compound Chinese herbal medicine best amount adding is about 1.2%. |
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| Keywords: | Pearl gentian grouper compound Chinese herbal medicine growth performance non-specific immunity digestive enzyme activity |
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