Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization |
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| Authors: | Zhan Wen-bin Wang Yuan-hong Zhang Zhi-dong Fukuda Hideo |
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| Institution: | (1) Aquaculture Research Laboratory, Ocean University of Qingdao, 266003 Qingdao, China;(2) Tokyo University of Fisheries, Konan, Minato, Tokyo, Japan |
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| Abstract: | White Spot Syndrome Virus (WSSV) was purified from hemolymph of infected shrimp. After nucleic acid extraction from the purified
virus particles, EcoR I-digested fragments of the WSSV genome were cloned; three of these fragments were used as non-radioactive
probes labeled with DIG-11-dUTP. The probes hybridized in situ, with sections located in the nuclei of all WSSV-infected tissues.
The virus was detected in the gill, stomach, epidermis, and connective tissue and so on, but not detected in healthy shrimp
tissues and epithelial cells of hepatopancreatic tubules of diseased shrimp.
This work was supported by SRF for ROCS, SEM and the National 863 Project (Grant 819-Q-08) and Project under major State Basic
Research Development Program (Grant G1999012002). |
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| Keywords: | WSSV Penaeus chinensis in situ hybridization |
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