| 基于16S rRNA序列初步探讨贻贝属的系统发育 |
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| 引用本文： | 沈玉帮,李家乐,牟月军.基于16S rRNA序列初步探讨贻贝属的系统发育[J].海洋科学,2009,33(12):50-55. |
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| 作者姓名： | 沈玉帮 李家乐 牟月军 |
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| 作者单位： | 1. 上海海洋大学,农业部水产种质资源与养殖生态重点开放实验室,上海,200090
2. 浙江省嵊泗县科学技术局,浙江,嵊泗,202450 |
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| 基金项目： | 国家"863"计划项目，上海市教委重点学科资助项目，上海市优青项目 |
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| 摘 要： | 通过比较贻贝属5个物种包括中国的两种贻贝的线粒体16S rRNA基因部分序列,来初步确定它们的系统发育关系和了解中国沿海两种贻贝的遗传多样性情况.以Perna viridis为外群,采用NJ法和MP法构建分子系统树.系统发育分析表明,5种贻贝(Mytilus californianus, M. corcuscus, M. galloprovincialis, M. edulis, M. trossulus)在系统树上依次进行分叉,呈放射状.M. californianus最为原始,M. corcuscus次之.每一个贻贝物种都形成单系.其中,M. edulis和M. trossulus是非常相似的,M. corcuscus和M. californianus的亲缘关系近.同时发现,我国沿海分布的紫贻贝(M. galloprovincialis)和厚壳贻贝(M. corcuscus)的遗传多样性都较高,但厚壳贻贝的遗传多样性要低于紫贻贝,可能是由于厚壳贻贝过度被渔民开采等导致厚壳贻贝群体大小降低的缘故.这里系统发育分析为将来进行物种进化、迁移和育种方面的比较研究提供理论基础.
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| 关 键 词： | 贻贝属 系统发育 单倍型 |
| 收稿时间： | 2008/10/16 0:00:00 |
| 修稿时间： | 2009/3/16 0:00:00 |
Molecular phylogenetics of genus Mytilus based on the 16S rRNA sequences |
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| SHEN Yu-bang,LI Jia-le and MU Yue-jun.Molecular phylogenetics of genus Mytilus based on the 16S rRNA sequences[J].Marine Sciences,2009,33(12):50-55. |
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| Authors: | SHEN Yu-bang LI Jia-le and MU Yue-jun |
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| Abstract: | In this paper we compared the partial sequences of the mitochondrial 16S ribosomal RNA of the five species of genus Mytilus including two species in China to primarily determine their phylogenetic relationships and estimate the genetic diversity of two species of Mytilus along the coast of China. The phylogenetic trees were reconstructed based on neighbor-joining and maximum parsimony methods. Perna viridis was used as an outgroup. The phylogenetic analysis revealed that five species of mussel Mytilus, M. californianus, M. corcuscus, M. galloprovincialis, M. edulis, M. trossulus, diverged in the basal polytomy at the base of the Mytilus radiation. M. californianus is the most basal among all species of genus M. ytilus, and the secondary is M. coruscus. The individuals of the each of five species solely cluster a monophyletic clade, respectively. M. edulis and M. trossulus are the most similar to the three closely related species. M. corcuscus is a closer relative of M. californianus than of M. edulis, M. galloprovincialis, M. trossulus. And we found that while the genetic diversity of both M. corcuscus and M. galloprovincialis distributed along the coast of China was higher, the genetic diversity of M. corcuscus was lower than that of M. galloprovincialis, this may be caused by declined population size due to overexploitation and human activity. This molecular phylogeny provides the theoretic foundation for future comparative studies of species evolution, immigration and breeding strategies. |
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| Keywords: | 16S rRNA Mytilus 16s ribosome RNA phylogeny haplotype |
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