| 保存液中Ca2+、Mg2+、K+对中华绒螯蟹（Eriocheir sinensis）精子体外保存存活率的影响 |
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| 引用本文： | 陈东华,李艳东,贾林芝,厉小波,王立人,孙菊香,王群.保存液中Ca2+、Mg2+、K+对中华绒螯蟹（Eriocheir sinensis）精子体外保存存活率的影响[J].海洋与湖沼,2007,38(6):585-589. |
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| 作者姓名： | 陈东华 李艳东 贾林芝 厉小波 王立人 孙菊香 王群 |
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| 作者单位： | 华东师范大学生命科学学院,上海,200062 |
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| 基金项目： | 国家自然科学基金资助项目,30300265号。 |
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| 摘 要： | 提要以伊红染色法检测样品精子存活率为依据,研究了4℃下5种保存液及Ca2 、Mg2 离子对中华绒螯蟹精子体外保存效果的影响。5种保存液分别为人工海水(ASW)、2倍钙离子人工海水(2×Ca2 -ASW)、无镁离子人工海水(Mg2 -FASW)、无钾离子人工海水(K -FASW)、无钙离子人工海水(Ca2 -FASW),经4天保存后,各保存液中精子样品的存活率和精子密度均出现明显差异,K -FASW、ASW及2×Ca2 -ASW三种保存液中的精子因发生顶体反应而大量死亡,而Mg2 -FASW、Ca2 -FASW的保存效果较好。在此基础上,进一步探讨了不同Mg2 和Ca2 浓度对精子存活率的影响,结果发现,经24h保存后各实验组精子存活率均随着两种离子浓度的增加而明显下降。上述结果表明:K -FASW、ASW及2×Ca2 -ASW不适合精子保存,而Mg2 -FASW和Ca2 -FASW均可作为该蟹精子的保存液;Ca2 因可引起精子顶体反应而造成保存液中精子的大量死亡,其浓度与存活率呈明显的负相关;无K 的保存液中,Ca2 、Mg2 的存在与否对精子的保存效果起关键作用;无Mg2 人工海水之所以具有较好的保存效果,可能与Mg2 的缺乏而导致Ca2 逆浓度差转运受阻,避免了因Ca2 进入而诱发顶体反应有关。
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| 关 键 词： | 中华绒螯蟹 精子 低温保存 保存液 Ca2 Mg2 K |
| 收稿时间： | 5/8/2006 12:00:00 AM |
| 修稿时间： | 2006年5月8日 |
THE VIABILITY RATE OF ERIOCHEIR SINENSIS SPERMS IN VITRO IN PRESERVATION SOLUTIONS OF Ca2+, Mg2+ AND K+ AT DIFFERENT CONCENTRATIONS |
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| CHEN Dong-Hu,LI Yan-Dong,JIA Lin-Zhi,LI Xiao-Bo,WANG Li-Ren,SUN Ju-Xiang and WANG Qun.THE VIABILITY RATE OF ERIOCHEIR SINENSIS SPERMS IN VITRO IN PRESERVATION SOLUTIONS OF Ca2+, Mg2+ AND K+ AT DIFFERENT CONCENTRATIONS[J].Oceanologia Et Limnologia Sinica,2007,38(6):585-589. |
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| Authors: | CHEN Dong-Hu LI Yan-Dong JIA Lin-Zhi LI Xiao-Bo WANG Li-Ren SUN Ju-Xiang and WANG Qun |
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| Institution: | School of Life Science, East China Normal University, Shanghai, 200062;School of Life Science, East China Normal University, Shanghai, 200062;School of Life Science, East China Normal University, Shanghai, 200062;School of Life Science, East China Normal University, Shanghai, 200062;School of Life Science, East China Normal University, Shanghai, 200062;School of Life Science, East China Normal University, Shanghai, 200062;School of Life Science, East China Normal University, Shanghai, 200062 |
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| Abstract: | The viability rate of in vitro sperms of Eriocheir sinensis preserved in five solutions at 4°C was studied with eosin stain to evaluate the impact from metal ions. The five preservation solutions (Tab.1) included artificial seawater (ASW), 2-fold calcium ion artificial seawater (2×Ca2+-ASW), magnesium-free artificial seawater (Mg2+-FASW), potassium-free artificial seawater (K+-FASW) and calcium-free artificial seawater (Ca2+-FASW). Results show that the sperms preserved in K+-FASW, ASW, and 2×Ca2+-ASW were completely killed 4 days later because of acrosome reaction. However, most of the sperms in Mg2+-FASW and Ca2+-FASW remained alive in the same period. Therefore, Mg2+-FASW and Ca2+-FASW can be ideal solutions for preserving E. sinensis sperms in vitro, but K+-FASW, ASW, and 2×Ca2+-ASW. Furthermore, the viability rate was also checked with different concentrations of Ca2+ and Mg2+. As the concentrations of Ca2+ and Mg2+ incremented, the viability rate dropped in 24 hour, indicating that Ca2+ could lead to mass mortality of the sperms due to acrosome reaction. Inverse correlation was found between the viability rate and Ca2+ concentration. Ca2+ and Mg2+ played a key role in e K+-free preservation solutions. The good performance of Mg2+-FASW is probably due to the prohibition of Ca2+ transportation against the concentration difference, so that Ca2+ was kept away from entering the sperms resulting in acrosome reaction. |
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| Keywords: | Eriocheir sinensis Spermatozoa Low temperature preservation Preservation solution Ca2 Mg2 K |
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