Differential gene expression in oyster exposed to sewage |
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| Authors: | Medeiros Igor Dias Siebert Marília Nardelli de Toledo e Silva Guilherme Moraes Milton Ozório Marques Maria Risoleta Freire Bainy Afonso Celso Dias |
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| Institution: | Departamento de Bioquímica, Centro de Ciências Biológicas, Campus Universitário, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil. |
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| Abstract: | In order to investigate the influence of domestic sewage on the gene expression of Pacific oysters Crassostrea gigas, suppression subtractive hybridization (SSH) method was employed. Oysters were sampled at a farming area and, after 10 days of acclimation in the laboratory, were exposed to untreated domestic sewage diluted 33% for 48 h. Gills of male oysters were excised for total RNA extraction. mRNA was purified and the differential gene expression was analyzed by SSH. We obtained 61 cDNA sequences but only 15 were identified, which includes fatty acid binding protein, multidrug resistance protein, omega glutathione S-transferase, cytochrome P450 isoform CYP356A1, among others. The identified genes are associated with different metabolic functions like biotransformation, membrane transport, aerobic metabolism and translational machinery, evidencing the potential toxic effects elicited by these effluents. |
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