| Non-specific immune response of bullfrog Rana catesbeiana to intraperitoneal injection of bacterium Aeromonas hydrophila |
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| 作者姓名： | 张俊杰 邹文政 鄢庆枇 |
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| 作者单位： | Fisheries College，Jimei University，Xiamen 361021，China，Chemistry and Chemical Engineering College of Xiamen University，Xiamen 361005，China |
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| 基金项目： | 国家高技术研究发展计划(863计划)，福建省自然科学基金 |
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| 摘 要： | Non-specific immune response of bullfrog Rana catesbeiana to pathogenic Aeromonas hydrophila was studied to 60 individuals in two groups.Each bullfrog in bacterium-injected group was injected intraperitoneally(i.p.) with 0.2 ml bacterial suspension at a density of 5.2 × 106 CFU/ml,while each one in control group injected i.p.with 0.2 ml sterile saline solution(0.85%,w/v).Three bullfrogs in both groups were sampled at 0,1,3,7,11,15 and 20 days post-injection(dpi) for the evaluation of non-specific immune parameters.It was observed that intraperitoneal injection of A.hydrophila significantly increased the number of leucocytes and that of NBT-positive cells in peripheral blood.Significant increases in serum bactericidal activity and serum acid phosphatase activity were also observed in the bacterium-injected frogs when compared with those in the control group.However,a significant reduction was detected in vitro in phagocytosis activity of peripheral blood phagocytes.No significant difference in changes in the number of peripheral erythrocytes,serum superoxide dismutase(SOD) activity,and lysozyme activity was detected between the two groups.It is suggested that bullfrogs may produce a series of non-specific immune reactions in response to the A.hydrophila infection.
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| 关 键 词： | 气单胞菌属 腹腔注入 免疫性反应 生物化学 |
Non-specific immune response of bullfrog Rana catesbeiana to intraperitoneal injection of bacterium Aeromonas hydrophila |
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| ZHANG Junjie,ZOU Wenzheng,YAN Qingpi Fisheries College,Jimei University,Xiamen ,China Chemistry and Chemical Engineering College of Xiamen University,Xiamen ,China.Non-specific immune response of bullfrog Rana catesbeiana to intraperitoneal injection of bacterium Aeromonas hydrophila[J].Chinese Journal of Oceanology and Limnology,2008,26(3). |
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| Authors: | ZHANG Junjie ZOU Wenzheng YAN Qingpi Fisheries College Jimei University Xiamen China Chemistry and Chemical Engineering College of Xiamen University Xiamen China |
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| Institution: | [1]Fisheries College, Jimei University; Xiamen 361021, China [2]Chemistry and Chemical Engineering College of Xiamen University, Xiamen 361005, China |
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| Abstract: | Non-specific immune response of bullfrog Rana catesbeiana to pathogenic Aeromonas hydrophila was studied to 60 individuals in two groups. Each bullfrog in bacterium-injected group was injected intraperitoneally (i.p.) with 0.2 ml bacterial suspension at a density of 5.2 × 106 CFU/ml, while each one in control group injected i.p. with 0.2 ml sterile saline solution (0.85%, w/v). Three bullfrogs in both groups were sampled at 0, 1, 3, 7, 11, 15 and 20 days post-injection (dpi) for the evaluation of non-specific immune parameters. It was observed that intraperitoneal injection of A. hydrophila significantly increased the number of leucocytes and that of NBT-positive cells in peripheral blood. Significant increases in serum bactericidal activity and serum acid phosphatase activity were also observed in the bacterium-injected frogs when compared with those in the control group. However, a significant reduction was detected in vitro in phagocytosis activity of peripheral blood phagocytes. No significant difference in changes in the number of peripheral erythrocytes, serum superoxide dismutase (SOD) activity, and lysozyme activity was detected between the two groups. It is suggested that bullfrogs may produce a series of non-specific immune reactions in response to the A. hydrophila infection. |
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| Keywords: | Rana catesbeiana Aeromonas hydrophila intraperitoneal injection non-specific immune response |
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