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| 大银鱼和小齿日本银鱼线粒体细胞色素b和16S rRNA基因部分序列分析 | |
| 引用本文： | 高天翔,陈省平,韩志强,刘进贤,张亚平.大银鱼和小齿日本银鱼线粒体细胞色素b和16S rRNA基因部分序列分析[J].中国海洋大学学报(自然科学版),2004,34(5):791-794. |
| 作者姓名： | 高天翔 陈省平 韩志强 刘进贤 张亚平 |
| 作者单位： | 1. 中国海洋大学,山东,青岛,266003 2. 中山大学,广东,广州,510275 3. 中国科学院昆明动物研究所,云南,昆明,650223 |
| 基金项目： | 国家重点基础研究发展规划项目 (G1 9990 4 37)资助 |
| 摘 要： | 对大银鱼和小齿日本银鱼的线粒体细胞色素b和16S rRNA基因片段进行了扩增和序列测定，分析比较了2种间的序列差异。大银鱼2个个体间细胞色素b基因序列无差异，小齿日本银鱼3个个体的序列出现了2种单倍型；大银鱼同小齿日本银鱼细胞色素b序列(单倍型SB-1)之间存在86个碱基差异(差异率21％)，变异较大。大银鱼、小齿日本银鱼的16S rRNA基因片段种内个体间无序列差异，两种间存在21个碱基的差异(差异率5％)，有1个碱基的插入／缺失。由此可见，2种银鱼间线粒体细胞色素b基因的进化速率较快，约为16S rRNA基因片段的4倍。根据细胞色素b序列数据。推算出2种银鱼大概在中新世晚期发生分化。 |
| 关 键 词： | 大银鱼 小齿日本银鱼 线粒体 细胞色素b 序列 |
| 文章编号： | 1672-5174(2004)05-791-04 |
| 修稿时间： | 2004年3月29日 |
Partial Sequence Analysis of Mitochondrial Cytochrome b and 16S rRNA Genes of Protosalanx hyalocranius and Salangichthys microdon |
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| GAO Tian-xiang,CHEN Sheng-ping,HAN Zhi-qiang,LIU Jin-xian,ZHANG Ya-ping.Partial Sequence Analysis of Mitochondrial Cytochrome b and 16S rRNA Genes of Protosalanx hyalocranius and Salangichthys microdon[J].Periodical of Ocean University of China,2004,34(5):791-794. | |
| Authors: | GAO Tian-xiang CHEN Sheng-ping HAN Zhi-qiang LIU Jin-xian ZHANG Ya-ping |
| Institution: | GAO Tian-xiang1,CHEN Sheng-ping2,HAN Zhi-qiang1,LIU Jin-xian1,ZHANG Ya-ping3 |
| Abstract: | The mitochondrial cytochrome b and 16S rRNA genes fragments of Protosalanx hyalocranius and Salangichthys microdon were amplified and sequenced. The cytochrome b sequence results showed that no sequence variation was observed in Protosalanx hyalocranius; there were two haplotypes in Salangichthys microdon samples. There were 86 different sites (21%) between P. hyalocranius and S. microdon (haplotype SB-1). No individual variation was observed in the partial sequences of 16S rRNA gene of P. hyalocranius or S. microdon. There were 21 different sites (5%) and one deletion/insertion occured between P. hyalocranius and S. microdon. This study suggests that the evolution rate of cytochrome b was four times that of 16S rRNA. The cytochrome b sequence divergence indicated that divergence of the two species occurred during the late Miocene. |
| Keywords: | Protosalanx hyalocranius Salangichthys microdon cytochrome b 16S rRNA sequence |
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