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| Design of Vibrio 16S rRNA Gene Specific Primers and Their Application in the Analysis of Seawater Vibrio Community | |
| 作者姓名： | LIU Yong YANG Guanpin WANG Hualei CHEN Jixiang SHI Xianming ZOU Guiwei WEI Qiwei SUN Xiuqin |
| 作者单位： | [1]College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P.R. China [2]Department of Food Science and Technology, Shanghai Jiaotong University, Shanghai 201101, P.R. China [3]Yangtse River Fishery Institute, Chinese Academy of Fishery, Jingzhou 434000, P.R. China [4]The First Institute of Oceanography, National Oceanic Administration of China, Qingdao 266061, P.R. China |
| 基金项目： | 中国科学院资助项目;科技部科研项目 |
| 摘 要： | The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene （rDNA） fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation. |
| 关 键 词： | 弧菌 细菌群落 海水 海洋微生物学 |
| 收稿时间： | 2004-11-29 |
| 修稿时间： | 2005-12-06 |
Design ofVibrio 16S rRNA gene specific primers and their application in the analysis of seawaterVibrio community |
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| LIU Yong YANG Guanpin WANG Hualei CHEN Jixiang SHI Xianming ZOU Guiwei WEI Qiwei SUN Xiuqin.Design of Vibrio 16S rRNA Gene Specific Primers and Their Application in the Analysis of Seawater Vibrio Community[J].Journal of Ocean University of China,2006,5(2):157-164. | |
| Authors: | Liu Yong Yang Guanpin Wang Hualei Chen Jixiang Shi Xianming Zou Guiwei Wei Qiwei Sun Xiuqin |
| Institution: | 1. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P.R. China 2. Department of Food Science and Technology, Shanghai Jiaotong University, Shanghai 201101, P.R. China 3. Yangtse River Fishery Institute, Chinese Academy of Fishery, Jingzhou 434000, P.R. China 4. The First Institute of Oceanography, National Oceanic Administration of China, Qingdao 266061, P.R. China |
| Abstract: | The pathogenic species of genus Vibrio cause vibriosis, one of the most prevalent diseases of maricultured animals and seafood consumers. Monitoring their kinetics in the chain of seafood production, processing and consumption is of great importance for food and mariculture safety. In order to enrich Vibrio-representing 16S ribosomal RNA gene (rDNA) fragments and identify these bacteria further real-timely and synchronously among bacterial flora in the chain, a pair of primers that selectively amplify Vibrio 16S rDNA fragments were designed with their specificities and coverage testified in the analysis of seawater Vibrio community. The specificities and coverage of two primers, VF169 and VR744, were determined theoretically among bacterial 16S rDNAs available in GenBank by using BLAST program and practically by amplifying Vibrio 16S rDNA fragments from seawater DNA. More than 88.3% of sequences in GenBank, which showed identical matches with VR744, belong to Vibrio genus. A total of 33 clones were randomly selected and sequenced. All of the sequences showed their highest similarities to and clustered around those of diverse known Vibrio species. The primers designed are capable of retrieving a wide range of Vibrio 16S rDNA fragments specifically among bacterial flora in seawater, the most important natural environment of seafood cultivation. |
| Keywords: | Vibrio vibriosis bacterial community primer |
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