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Application of RAPD technology for identification in three different stocks of Penaeus chinensis
作者姓名:Liu Zhenhui  Kong Jie  Liu Ping  Deng Jingyao
作者单位:Liu Zhenhui1,Kong Jie 2,Liu Ping2,Deng Jingyao2 1. College of Marine Biology Science,Ocean University of China,Qingdao 266003,China 2. Huanghai Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,266071,China
摘    要:INTRODUCTIONPenaeuschinensishasbecomeoneoftheprimaryspeciesforaquacultureinChina .How ever ,viralandbacterialdiseaseproblemshaveplaguedtheshrimpaquacultureindustryserious lyinChinasince 1 993.Ithasbeenindicatedthatthereducedgrowthperformanceandthein creasedsusceptibilitytodiseasescouldbearesultoflowlevelsofgeneticvariablities (Carretal .,1 994 ) .InordertosupporttheaquacultureindustryoftheUnitedStates,theUSMarineShrimpFarmaringProgramConsortiumhasdevelopedvariousdisease free populati…

关 键 词:Penaeus  chinensis  RAPD  technology  genetic  marker
收稿时间:4/8/2003 12:00:00 AM
修稿时间:2003/6/12 0:00:00

Application of RAPD technology for identification in three different stocks of Penaeus chinensis
Liu Zhenhui,Kong Jie,Liu Ping,Deng Jingyao.Application of RAPD technology for identification in three different stocks of Penaeus chinensis[J].Acta Oceanologica Sinica,2003,22(4):629-634.
Authors:Liu Zhenhui  Kong Jie  Liu Ping and Deng Jingyao
Institution:1.College of Marine Biology Science, Ocean University of China, Qingdao 266003, China2.Huanghai Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China
Abstract:The genetic structure of three stocks (K, H and S) of Penaeus chinensis was detected by RAPD method which useed 41 random oligonucleotide primers with 10 bases. Stock K was from the spawning stock of the western coastal waters of the Korea Peninsula, H was from the Huanghai SeaBohai Sea stock and S was from the health cultured stock in Jimo, Shandong Province, China. Three genetic markers, F- 02540, OPN - 13800 and OPN - 202200, which could differentiate the three different stocks, have been reported. The basic data for marker assisted selection (MAS) on genetic breeding of Penaeus chinensis are provided.
Keywords:Penaeus chinensis  RAPD technology  genetic marker
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