Phosphate-free ornithine lipid contents in Desulfovibrio spp. respond to growth temperature |
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| Institution: | 1. Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University of Oldenburg, Carl-von-Ossietzky-Str. 9-11, 26129 Oldenburg, Germany;2. Max Planck Research Group for Marine Geochemistry, ICBM, Carl von Ossietzky University of Oldenburg, Carl-von-Ossietzky-Str. 9-11, 26129 Oldenburg, Germany;3. School of Earth and Ocean Sciences, Cardiff University, Cardiff CF10 3YE, Wales, UK;1. Cornell University Department of Physics, 117 Clark Hall, Ithaca, New York, 14853, United States;2. Johns Hopkins University Department of Molecular Biophysics, 101 Jenkins Hall, 3400 N. Charles Street, Baltimore, Maryland, 21218, United States;3. Georgia Institute of Technology Partnership for an Advanced Computing Environment, 756 W. Peachtree St. NW, Atlanta, Georgia, 30332, United States;4. Cornell University Department of Molecular Biology and Genetics, Room 201 215 Tower Rd. Ithaca, New York, 14853, United States;1. WA Organic and Isotope Geochemistry Centre, Department of Earth and Planetary Sciences, Curtin University, WA, Australia;2. School of Earth Sciences, University of Western Australia, WA, Australia;1. Istanbul University, Faculty of Engineering, Department of Environmental Engineering, Istanbul, Turkey;2. Firat University, Faculty of Engineering, Department of Environmental Engineering, Elazig, Turkey;1. State Key Laboratory of Petroleum Resources and Prospecting, China University of Petroleum (Beijing), Beijing 102249, China;2. SINPEC Zhongyuan Oilfield Company, Puyang, Henan 457001, China;3. Department of Earth and Planetary Sciences, Curtin University, Perth, WA 6845, Australia;4. School of Earth Sciences, University of Western Australia, Perth, WA 6009, Australia;1. International Postgraduate Programs in Environmental Management, Graduate School, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand;2. Center of Excellence on Hazardous Substance Management (HSM), Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand;3. Faculty of Environmental Management, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;4. Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand;5. Department of Civil and Environmental Engineering, College of Engineering, North Dakota State University, Fargo, ND 58108, USA;1. Applied Petroleum Technology AS, P.O. Box 123, 2027 Kjeller, Norway;2. Lundin Norway AS, Strandveien 50 B, 1366 Lysaker, Norway |
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| Abstract: | Eight sulfate-reducing Desulfovibrio strains isolated from intertidal sediments of the North Sea were investigated for their intact polar lipid (IPL) composition. They contained two types of IPLs, phospholipids and aminolipids. The dominating phospholipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and, in lower concentration, diphosphatidylglycerol (DPG). Aminolipids formed a significant IPL fraction in all strains and high resolution tandem mass spectrometry assigned them as phosphate-free ornithine lipids (OLs). In intertidal flat sediments microorganisms may face strong temperature change on varying timescales and it is crucial for the microbes to maintain constant membrane fluidity, e.g. by modification of their membrane lipid composition. We therefore investigated whether or not these strains employ the same strategies for adjusting their membrane composition to growth temperature and, in particular, how OLs are modified. In all strains the relative OL content was found to be higher at higher growth temperature, in most cases at the expense of PE content and less often PG content. The fatty acid (FA) side chains of the main PE and PG species were similar, i.e. both were dominated by C17 or C18, whereas C15 FAs were additionally found as major OL side chains. The temperature-related side chain variation was similar for all IPLs: unsaturated FA content was lower at higher temperature. The corresponding FA patterns after hydrolysis revealed elevated branched FA content and anteiso/iso ratio at higher growth temperature. As the temperature-related changes in the IPL side chains were similar for all strains, we conclude that side chain modification plays a major role in the maintenance of membrane fluidity at higher temperature and that alternative roles of OLs in the membrane adaptation of Desulfovibrio spp. other than melting point adjustment are possible. |
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