An automated purification method for archaeal and bacterial tetraethers in soils and sediments |
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| Institution: | 1. Institute of Lighting and Energy Photonics, National Chiao-Tung University, Tainan 71150, Taiwan;2. Institute of Photonic System, National Chiao-Tung University, Tainan 71150, Taiwan;3. Department of Biomedical Engineering, Hungkuang University, Taichung City, 43302, Taiwan;4. Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan;1. School of Physics, State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China;2. Department of Physics, Jining University, Qufu, 273155, China;3. School of Information Science and Engineering, Shandong University, Jinan, 250100, China;1. School of Science, Hunan University of Technology, Zhuzhou, 412007, China;2. Hunan Key Laboratory of Super Micro-structure and Ultrafast Process, Central South University, Changsha, 410083, China;1. State Key Laboratory of Biogeology and Environmental Geology, School of Earth Sciences, China University of Geosciences, Wuhan 430074, China;2. Organic Geochemistry Unit, Bristol Biogeochemistry Research Centre and The Cabot Institute, School of Chemistry, University of Bristol, Cantock’s Close, Bristol BS8 1TS, UK;1. Department of Environmental Sciences, University of Basel, Bernoullistrasse 30, 4056 Basel, Switzerland;2. Department of Marine Organic Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, P.O. Box 59, 1790 AB Den Burg, Texel, The Netherlands;3. Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Surface Waters – Research and Management, Seestrasse 79, 6047 Kastanienbaum, Switzerland;4. Utrecht University, Faculty of Geosciences, P.O. Box 80.021, 3508 TA Utrecht, The Netherlands |
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| Abstract: | Isoprenoid and branched glycerol dialkyl glycerol tetraethers (GDGTs) are archaeal and bacterial polar lipids increasingly used as environmental biomarkers, and are studied in a wide range of settings: lacustrine and oceanic sediments, water column particulate organic matter, soils, peats, sedimentary rocks and extracts from archaeal and bacterial cultures. In paleoclimatology, for example, typical work on a sediment core of several tens of m consists of several hundreds to more than a thousand HPLC–MS (high performance liquid chromatography–mass spectrometry) analyses. The measurements therefore require purification steps from total lipid extracts. We propose an automated procedure for obtaining the GDGT core lipid fraction. We first evaluate both the yield and efficiency of the separation using different cartridges. We then compare the results from automated and “classical manual” procedures for a soil and a marine sediment, as well as for a sedimentary paleosequence. |
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