o,p′-DDT induction of vitellogenesis and its inhibition by tamoxifen in Nile tilapia (Oreochromis niloticus) |
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| Authors: | Olga Leaos-Castaeda Glen Van Der Kraak Andrea Lister Raúl Sim-Alvarez Gerardo Gold-Bouchot |
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| Institution: | Olga Leaños-Castañeda, Glen Van Der Kraak, Andrea Lister, Raúl Simá-Alvarez,Gerardo Gold-Bouchot |
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| Abstract: | In order to investigate the mechanism by which o,p′-DDT disrupts endocrine functioning of Nile tilapia in vivo, the estrogenicity of o,p′-DDT was investigated in conjunction with 17β-estradiol (E2) and tamoxifen. Mature, male tilapia were treated intraperitoneally with o,p′-DDT (60 mg/kg, one dose) or E2 (5 mg/kg, four doses) in the presence or absence of tamoxifen (5 mg/kg, six doses) for 12 days and then plasma vitellogenin (Vtg) (measured as alkaline-labile phosphorous), E2, and testosterone (T) were measured. Vtg levels were increased dramatically by E2 (1744±171 μg/ml) and moderately by o,p′-DDT (82±15 μg/ml) compared with controls (23±3.5 μg/ml). Tamoxifen alone had no effect on Vtg production, but inhibited both E2 and o,p′-DDT stimulated vitellogenesis. T levels were reduced with E2 administration (1688±383 pg/ml) and declined further with the combined treatment of E2 and tamoxifen (281±70 pg/ml), compared with controls (6558±1438 pg/ml). Tamoxifen or o,p′-DDT alone did not affect T levels, but their combined treatment did (2069±647 pg/ml). The results of this study suggest that o,p′-DDT is weakly estrogenic in male tilapia, and that this activity may be mediated through the estrogen receptor. |
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| Keywords: | DDT Tamoxifen Vitellogenin Estradiol Testosterone Tilapia Fish Endocrine disruption Antiestrogen |
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