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中国对虾家系建立及不同家系生长发育的初步研究
引用本文:张天时,孔杰,刘萍,王清印,张庆文.中国对虾家系建立及不同家系生长发育的初步研究[J].海洋学报,2007,29(3):120-124.
作者姓名:张天时  孔杰  刘萍  王清印  张庆文
作者单位:中国水产科学研究院, 黄海水产研究所, 农业部海洋渔业资源可持续利用重点开放实验室, 山东, 青岛, 266071
基金项目:山东省自然科学基金 , 国家高技术研究发展计划(863计划) , 中国-挪威合作项目
摘    要:在中国对虾(Fenneropenaeus chinensis)家系建立过程中,我们采用定向交配对子代进行选育的方法.采用4种中国对虾人工授精方法,即整个精荚移植、精荚切块移植、输精管移植和精液移植,共进行了155例雌虾的人工授精实验,有108尾雌虾成功产卵并孵化出仔虾,成功率为69.7%,建立了101个全同胞家系和29个半同胞家系.在受精卵孵化、育苗的基础上,同时进行了不同家系幼体生长情况的研究,结果表明受精率、孵化率、存活率以及初期生长在4个实验组间都没有显著性差异.另外对其他家系之间的比较也得出同样的结果,说明不同家系在早期的生长中,没有表现出显著性差异.而在养成阶段,方差分析结果表明家系间在生长上表现出差异极其显著.

关 键 词:中国对虾(Fenneropenaeuschinensis)    家系    人工授精    移植    生长
文章编号:0253-4193(2007)03-0120-05
收稿时间:2005/12/27 0:00:00
修稿时间:2005-12-272006-07-12

Preliminary study of establishment of families and their growth and development for Fenneropenaeus chinensis
ZHANG Tian-shi,KONG Jie,LIU Ping,WANG Qing-yin and ZHANG Qing-wen.Preliminary study of establishment of families and their growth and development for Fenneropenaeus chinensis[J].Acta Oceanologica Sinica (in Chinese),2007,29(3):120-124.
Authors:ZHANG Tian-shi  KONG Jie  LIU Ping  WANG Qing-yin and ZHANG Qing-wen
Institution:Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
Abstract:Chinese fleshy prawn (Fennerop enaeus chinensis) families were produced by carrying out oriented mating so as to select the off springs in selective breeding of F.chinensis.We used 4 different methods of artificial insemination (AI),namely the entire spermatophores transplantation,the fractional spermatophores transplantation,the sperm duct transplantation and the seminal fluid transplantation.155 cases of artificial insemination experiment were totally applied,108 spaw ners succeeded in spaw ning and hatching the larvae,with 69.7% of the ratio.101 full-sib families that included 29 half-sib families of F.chinensis were established.The larval growth and development were studied,such as fertilization rate,hat ching rate,survival rate and growth were compared.There were nosignificant differences among four different experimental groups (RS(♂)×RS(♀),RS(♂)×QD(♀),RS(♂)×KC(♀) and QD(♂)×KC(♀)).In addition,there were no significant differences between other families that were studied in the experiment at early life history stage of F.chinensis.During growout,analyses of variance for growth indicated there was most significant difference among families.
Keywords:Fenneropenaeus chinensis  families  artificial insemination  transplantation  growth
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