Optimization of the purification methods for recovery of recombinant growth hormone from Paralichthys olivaceus |
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| Authors: | ZANG Xiaonan ZHANG Xuecheng MU Xiaosheng LIU Bin |
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| Institution: | 1. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P.R. China
2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, P.R. China |
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| Abstract: | This study aimed to optimize the purification of recombinant growth hormone from Paralichthys olivaceus. Recombinant flounder growth hormone (r-fGH) was expressed by Escherichia coli in form of inclusion body or as soluble protein under different inducing conditions. The inclusion body was renatured using two recovery methods, i.e., dilution and dialysis. Thereafter, the refolded protein was purified by Glutathione Sepharase 4B affinity chromatography and r-fGH was obtained by cleavage of thrombin. For soluble products, r-fGH was directly purified from the lysates by Glutathione Sepharase 4B affinity chromatography. ELISA-receptor assay demonstrated that despite its low receptor binding activity, the r-fGH purified from refolded inclusion body had a higher yield (2.605 mg L?1) than that from soluble protein (1.964 mg L?1). Of the tested recovery methods, addition of renaturing buffer (pH 8.5) into denatured inclusion body yielded the best recovery rate (17.9%). This work provided an optimized purification method for high recovery of r-fGH, thus contributing to the application of r-fGH to aquaculture. |
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| Keywords: | Paralichthys olivaceus growth hormone fusion expression purification receptor binding activity |
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