Tissue culture of three species of Laurencia complex |
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| Authors: | Songdong Shen Xunjian Wu Binlun Yan Lihong He |
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| Institution: | (1) LERVEM, University of New Caledonia, BP R4, 98851 Noumea cedex, New Caledonia, France |
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| Abstract: | To establish a micropropagation system of three Laurencia complex species (Laurencia okamurai, Laurencia tristicha, and Chondrophycus undulatus) by tissue culture techniques, we studied the regeneration characteristics and optimal culture conditions of axenic algal
fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under
a reverse microscope to investigate the regeneration process, polarity and optimal illumination, and temperature and salinity
levels. The results show that in most cultures of the three species, we obtained bud regeneration on solidified medium with
0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments
in L. okamurai, but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species, and on fragments cut from the
basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary
and the cortical portions in L. okamurai and C. undulatus, while in L. tristicha, buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx, 20°C and 35 (salinity); for C. undulatus, 4 500 lx, 20°C and 30; and for L. tristicha, 4 500 lx, 25°C and 30. |
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