Microbial diversity in contaminated soils along the T22 trench of the Chernobyl experimental platform |
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| Authors: | Virginie Chapon Laurie Piette Marie-Hélène Vesvres Frédéric Coppin Claire Le Marrec Richard Christen Nicolas Theodorakopoulos Laureline Février Sviatoslav Levchuk Arnaud Martin-Garin Catherine Berthomieu Claire Sergeant |
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| Institution: | 1. CEA, DSV, IBEB, SBVME, LIPM, F-13108 Saint-Paul-lez-Durance, France;2. CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance, France;3. Université d’Aix-Marseille, F-13108 Saint-Paul-lez-Durance, France;4. Université de Bordeaux 1/CNRS-IN2P3, UMR 5797, CENBG, POB 120, F-33175 Gradignan cedex, France;5. IRSN, DEI/SECRE/LRE-Bât 186, B.P.3, Cadarache Center, F-13115 Saint-Paul-lez-Durance cedex, France;6. ISVV, UMR 1219, Institut Polytechnique de Bordeaux/INRA, POB 50008, F-33882 Villenave d’Ornon, France;g Université de Nice-Sophia-Antipolis, Centre de Biochimie, Parc Valrose, F-06108 Nice, France;h CNRS, UMR 6543, Centre de Biochimie, Parc Valrose, F-06108 Nice, France;i Ukrainian Institute of Agricultural Radiology, UIAR, NUBiP of Ukraine, Mashinobudivnykiv Str. 7, Chabany, Kyiv-Svjatoshin Distr., Kyiv Reg. 08162, Ukraine |
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| Abstract: | The diversity of bacterial communities exposed to radioactive contamination in Chernobyl soils was examined by a combination of molecular and culture-based approaches. A set of six radioactive soil samples, exhibiting high levels of 137Cs contamination, were collected from the T22 trench. Three samples were also collected in nearby soils with low contamination. Complex bacterial community structures were observed in both highly and weakly contaminated samples, using a molecular approach targeting the 16S rRNA gene. However, the presence of specific populations within samples from highly contaminated soils could not be revealed by statistical analysis of the DGGE profiles. More than 200 culturable isolates, representative of dominant morphotypes, were grouped into 83 Operational Taxonomic Units (OTUs) and affiliated to Firmicutes, Actinobacteria, Alpha-, Beta-, Gamma-Proteobacteria and Bacteroïdetes. No specific pattern linked to contamination was observed for these culturable bacteria. The results show that both highly and weakly contaminated soils host a wide diversity of bacteria, suggesting that long term exposure to radionuclides does not lead to the extinction of bacterial diversity. |
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