| PAEs类环境激素在草鱼（Ctenopharyngodonidellus）脏器中代谢研究 |
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| 引用本文： | 葛 建,李明揆,林 芳,赵 进,韩宝瑜.PAEs类环境激素在草鱼（Ctenopharyngodonidellus）脏器中代谢研究[J].海洋与湖沼,2011,42(4):549-553. |
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| 作者姓名： | 葛 建 李明揆 林 芳 赵 进 韩宝瑜 |
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| 作者单位： | 中国计量学院生命科学学院;浙江省生物计量及检验检疫技术重点实验室;中国计量学院生命科学学院;中国计量学院生命科学学院;中国计量学院生命科学学院;浙江省生物计量及检验检疫技术重点实验室 |
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| 基金项目： | 国家自然科学基金资助项目, 31100499 号; 浙江省自然科学基金资助项目, Y3080255 号、3100375 号; 浙江省分析测试计划项目, 2011C37044号 |
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| 摘 要： | 利用GC-FID建立了草鱼血清、肝胰脏、小肠及肝细胞培养液中邻苯二甲酸二乙酯（DEP）、邻苯二甲酸二丁酯（DBP）、邻苯二甲酸丁基苄酯（BBP）和邻苯二甲酸二乙基己基酯（DEHP）同时检测方法，并利用LC-MS／MS方法进行了DEP、DBP、BBP及DEHP在草鱼脏器中的代谢规律研究，以揭示邻苯二甲酸酯类（Phthal...
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| 关 键 词： | 草鱼 邻苯二甲酸酯类 气相色谱 液相色谱 质谱联用 |
| 收稿时间： | 2010/12/15 0:00:00 |
| 修稿时间： | 2011/2/21 0:00:00 |
METABOLISM OF PHTHALIC ACID ESTERS IN CTENOPHARYNGODON IDELLUS ORGANS AND HEPATOCYTE |
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| GE Jian,LI Ming-Kui,LIN Fang,ZHAO Jin and HAN Bao-Yu.METABOLISM OF PHTHALIC ACID ESTERS IN CTENOPHARYNGODON IDELLUS ORGANS AND HEPATOCYTE[J].Oceanologia Et Limnologia Sinica,2011,42(4):549-553. |
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| Authors: | GE Jian LI Ming-Kui LIN Fang ZHAO Jin and HAN Bao-Yu |
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| Institution: | College of Life Sciences, China Jiliang University;Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University;College of Life Sciences, China Jiliang University;College of Life Sciences, China Jiliang University;College of Life Sciences, China Jiliang University;Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University |
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| Abstract: | In order to reveal the toxicity mechanism of PAEs chemicals, the article developed a method for simultaneous determination of diethyl phthalate (DEP), dibutyl phthalate (DBP), butyl benzyl phthalate (BBP) and di-(2-ethylhexyl) phthalate (DEHP) by using GC-FID. Meanwhile, the metabolism of four PAEs chemicals was also studied in Ctenopharyngodon idellus organs and hepatocyte through LC-MS/MS. The results showed that PAEs chemicals were not metabolized significantly in hepatocyte, but they were metabolized quickly in blood serum, intestinal tract and hepato-pancreas homogenate, especially in the serum. And the main metabolites were mono-phthalates, including monoethyl phthalate (mEP), monobutyl phthalate (mBP), monobenzyl phthalate (mBzP) and monoethylhexyl phthalate (mEHP). Therefore, it was concluded that the PAEs chemicals were mainly hydrolyzed by means of the proteolytic enzymes in C. idellus organs, and the blood and intestine were the first metabolized location of PAEs chemicals absorbed by the fish gill and intestine. |
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| Keywords: | Ctenopharyngodon idellus Phthalic acid esters GC LC-MS/MS |
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