| Construction of a Metagenomic DNA Library of Sponge Symbionts and Screening of Antibacterial Metabolites |
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| 作者姓名： | CHEN Juan ZHU Tianjiao LI Dehai CUI Chengbin FANG Yuchun LIU Hongbing LIU Peipei GU Qianqun ZHU Weiming |
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| 作者单位： | [1]Key Laboratory of Marine Drugs of Chinese Ministry of Education, Institute of Marine Drug and Food,Ocean University of China, Qingdao 266003, P. R. China [2]Beijing Institute of Pharmacology and Toxicology, AMMS, Beijing 100850, P.R. China |
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| 基金项目： | 国家高技术研究发展计划(863计划);中国科学院资助项目;山东省自然科学基金 |
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| 摘 要： | To study the bioactive metabolites produced by sponge-derived uncultured symbionts, a metagenomic DNA library of the symbionts of sponge Gelliodes gracilis was constructed. The average size of DNA inserts in the library was 20 kb. This library was screened for antibiotic activity using paper disc assaying. Two clones displayed the antibacterial activity against Micrococcus tetragenus. The metabolites of these two clones were analyzed through HPLC. The result showed that their metabolites were quite different from those of the host E. coli DH5α and the host containing vector pHZ132. This study may present a new approach to exploring bioactive metabolites of sponge symbionts.
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| 关 键 词： | 海绵 共生体 生物活性 代谢物 生物化学 |
| 收稿时间： | 2005-11-28 |
| 修稿时间： | 2006-03-02 |
Construction of a metagenomic DNA library of sponge symbionts and screening of antibacterial metabolites |
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| CHEN Juan ZHU Tianjiao LI Dehai CUI Chengbin FANG Yuchun LIU Hongbing LIU Peipei GU Qianqun ZHU Weiming.Construction of a Metagenomic DNA Library of Sponge Symbionts and Screening of Antibacterial Metabolites[J].Journal of Ocean University of China,2006,5(2):119-122. |
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| Authors: | Chen Juan Zhu Tianjiao Li Dehai Cui Chengbin Fang Yuchun Liu Hongbing Liu Peipei Gu Qianqun Zhu Weiming |
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| Institution: | 1. Key Laboratory of Marine Drugs of Chinese Ministry of Education, Institute of Marine Drug and Food,Ocean University of China, Qingdao 266003, P. R. China
2. Beijing Institute of Pharmacology and Toxicology, AMMS, Beijing 100850, P.R. China |
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| Abstract: | To study the bioactive metabolites produced by sponge-derived uncultured symbionts, a metagenomic DNA library of the symbionts of sponge Gelliodes gracilis was constructed. The average size of DNA inserts in the library was 20 kb. This library was screened for antibiotic activity using paper disc assaying. Two clones displayed the antibacterial activity against Micrococcus tetragenus. The metabolites of these two clones were analyzed through HPLC. The result showed that their metabolites were quite different from those of the host E. coli DNA and the host containing vector pHZ132. This study may present a new approach to exploring bioactive metabolites of sponge symbionts. |
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| Keywords: | metagenomic DNA library sponge symbionts metabolite bioactivity |
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