Optimizing alginate beads for the immobilisation of Phaeodactylum tricornutum in estuarine waters |
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| Institution: | 1. KU Leuven Kulak, Research Unit Food & Lipids, E. Sabbelaan 53, 8500 Kortrijk, Belgium;2. Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven, Kasteelpark Arenberg 20, 3001 Leuven, Belgium;3. KU Leuven, Department of Biosystems, MeBioS division, Kasteelpark Arenberg 30, 3001 Leuven, Belgium;4. University of Antwerp, Department of Engineering Management, Prinsstraat 13, 2000 Antwerpen, Belgium;5. Technologiecluster Bioengineering Technology, Campus Brugge, Spoorwegstraat 12 - bus 7913, 8200 Sint-Michiels, Belgium;6. Departement Microbiële en Moleculaire Systemen, KU Leuven, Kasteelpark Arenberg 23 - bus 246, 3001 Leuven, Belgium;7. KU Leuven Kulak, Laboratory Aquatic Biology, E. Sabbelaan 53, 8500 Kortrijk, Belgium;1. University of South Florida College of Marine Science, 140 7th Ave S, St Petersburg, FL 33701, United States;2. Natural Sciences, Baruch College and Graduate Center, City University of New York, Box A-506, 17 Lexington Avenue, New York, NY 10010, United States;3. Florida State University Coastal & Marine Laboratory, 3618 Coastal Highway 98, St. Teresa, FL 32358, United States |
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| Abstract: | This study addresses the influence of calcium as hardening agent, on alginate gel bead stability and suitability for the growth of Phaeodactylum tricornutum Bohlin (Bacillariophyceae) in estuarine waters. Alginate beads produced with 1, 2, 4, 5 and 6% of CaCl2 solutions were investigated for stability and suitability for growth of P. tricornutum cells, under mean salinity 27, at 220 and 440 rpm stirring laboratory conditions, and in devices placed under in situ estuarine conditions. Gel stability and suitability for cell growth were evaluated through bead diameter, bead disruption, dissolution and loss of spherical shape, cell viability and specific growth rates. Beads gelled with 5% CaCl2 were found the most suitable to sustain gel stability and cell growth in the estuarine waters. These beads were surveyed during dredging operations in the Tagus estuary, both in situ and in estuarine water under laboratory conditions, showing significantly lowered growth rates possibly due to Mn, Co and As accumulated in the cells. Results confirmed that the monitoring tool presented is reliable and effective for the assessment of anthropogenic impacts. |
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| Keywords: | Cell immobilisation Hardening gel agent Monitoring tool Water quality Estuary |
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