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卵磷脂对花尾胡椒401幼鱼谷肤甘肤一硫一转移酶,谷肤甘肤过氧化物酶和DNA完整性的影响
引用本文:董宏坡,陈彦,谢仰杰,王重刚.卵磷脂对花尾胡椒401幼鱼谷肤甘肤一硫一转移酶,谷肤甘肤过氧化物酶和DNA完整性的影响[J].海洋科学,2004,28(4):25-28.
作者姓名:董宏坡  陈彦  谢仰杰  王重刚
作者单位:1. 厦门大学,生命科学学院,福建,厦门,361005
2. 集美大学,水产学院,福建,厦门,361021
基金项目:福建省自然科学基金资助项目(139910007)
摘    要:采用不同卵磷脂添加量的配合饲料投喂花尾胡椒鲷(Plectorhyncchus cinctus)幼鱼,同步测定幼鱼的谷胱甘肽-硫-转移酶和谷胱甘肽过氧化物酶活性以及DNA完整程度。结果表明,谷胱甘肽-硫-转移酶和谷胱甘肽过氧化物酶活性随着饲料中卵磷脂含量的增加而提高,而DNA单链断裂程度随着饲料中卵磷脂含量的增加而减少。这提示卵磷脂能够提高鱼体的抗氧化防御系统的机能。

关 键 词:卵磷脂  花尾胡椒鲷  幼鱼  谷胱甘肽-硫-转移酶  谷胱甘肽过氧化物酶  DNA完整性  饲料
文章编号:1000-3096(2004)04-0025-04
收稿时间:2003/3/15 0:00:00
修稿时间:2003年3月15日

Effect of lecithin on the glutathione S-transferase,glutathione pheroidase activities and DNA integrity of juvenile Plectorynchus cinctus
DONG Hong-po,CHEN Yan,XIE Yang-jie,WANG Chong-gang.Effect of lecithin on the glutathione S-transferase,glutathione pheroidase activities and DNA integrity of juvenile Plectorynchus cinctus[J].Marine Sciences,2004,28(4):25-28.
Authors:DONG Hong-po  CHEN Yan  XIE Yang-jie  WANG Chong-gang
Institution:DONG Hong-po1,CHEN Yan1,XIE Yang-jie2,WANG Chong-gang1
Abstract:The experiment was conducted to determine the effects of supplemental lecithin in the diet on juvenile Plectorhynchus cinctus. Five isocaloric diets were formulated by the addition of different levels (0,2,4,6,8g/100g diet) of soybean lecithin. The diets were fed to the five groups of juvenile Plectorhynchus cinctus for ten days. The glutathione S- transferase (GST) and glutathione pheroidase (GPx) activities, DNA integrity of juvenile Plectorhynchus cinctus were as- sayed simultaneously. The results indicated that the GST and GPx activities were elevated with increasing of lecithin levels in the diets, the extent of DNA single breaks decreased with the increasing of lecithin levels in the diets. This suggested that lecithin would enhance the function of the antioxidant defend system in the fish.
Keywords:lecithin  glutathione S-transferase  glutathione  pheroidase  DNA  integrity  Plectorhynchuscinctus
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