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The relationships between particulate and soluble alkaline phosphatase activities and the concentration of phosphorus dissolved in the seawater of Toulon Bay (NW Mediterranean)
Authors:Gérard Bogé  Magali Lespilette  Dominique Jamet  Jean-Louis Jamet
Institution:Université du Sud Toulon-Var, PROTEE, EA 3819, 83957 La Garde, France
Abstract:The activities of particulate and soluble phosphatase were analyzed monthly for 1 year in the coastal ecosystems of the NW Mediterranean Sea. The mean contribution of the particulate activity increased from 56% at an MUF-P concentration of 30 μM to 77% at 0.04 μM. This particulate activity was negatively correlated with the DIP, DOP and TDP concentrations when the activities were related to the seawater volume, chlorophyll a or the protein concentration. The TDP correlations were highly significant (p: 0.001). The DOP correlations were significant (p: 0.04) and became highly significant (p: 0.009) at low DIP concentrations (<0.13 μM). The DIP correlations were significant (p: 0.04) only at low DOP concentrations (<0.18 μM). Thus, the effects of seawater DIP and DOP were found to be linked. The soluble activity exhibited distinct phosphatase fractions with high (0.5–29.5 μM) and low (0.02–2 μM) Km values, but none exhibited significant correlations with phosphorus compounds.
Keywords:DIP  dissolved inorganic phosphorus  DOP  dissolved organic phosphorus  TDP  total dissolved phosphorus  SRP  soluble reactive phosphorus  MUF-P  Methyl umbelliferyl phosphate  MUF  methylumbelliferone
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