Time-course evaluation of ROS-mediated toxicity in mussels, Mytilus galloprovincialis, during a field translocation experiment |
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| Authors: | Giada Frenzilli Raffaella Bocchetti Massimiliano Pagliarecci Marco Nigro Francesca Annarumma Vittoria Scarcelli Daniele Fattorini Francesco Regoli |
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| Institution: | a Dipartimento di Morfologia Umana e Biologia Applicata, Università di Pisa, Italy;b Instituto di Biologia e Genetica, Università Politecnica delle Marche, Via Ranieri Monte D'Ago, 60100, Ancona, Italy |
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| Abstract: | Harbours can be considered as model environments for developing and validating field monitoring procedures and to investigate mechanistic relationships between different biological responses. In this study several biomarkers were investigated in marine mussels caged for 4 weeks (June–July 2001) into an industrialized harbour of NW Italy. Organisms were collected at different time-intervals to better characterize the sensitivity, temporal variations and interactions of analysed responses. Besides single antioxidants the total oxyradical scavenging capacity (TOSC) assay was used to analyse the capability of the whole antioxidant system to neutralize specific forms of radicals: these data were further integrated by measurement of DNA integrity, oxidized bases and the impairment of lysosomal membrane stability in haemocytes. Results showed a biphasic trend for single antioxidants and TOSC, with an increase during the first 2 weeks of exposure to the polluted site followed by a progressive decrease up to a severe depletion in the final part of the experiment. |
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| Keywords: | Mussels Biomonitoring Oxyradicals TOSC Lysosomes DNA damages |
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