|
|||||
|
|
Subcellular distribution of trace elements in the liver of sea turtles |
|
| Authors: | Anan Yasumi Kunito Takashi Sakai Haruya Tanabe Shinsuke |
| Institution: | a Center for Marine Environmental Studies (CMES), Ehime University, Tarumi 3-5-7, Matsuyama 790-8566, Japan b Department of Hygiene, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 230-0004, Japan |
| Abstract: | Subcellular distribution of Cu, Zn, Se, Rb, Mo, Ag, Cd and Pb was determined in the liver of green turtles (Chelonia mydas) and hawksbill turtles (Eretmochelys imbricata) from Yaeyama Islands, Japan. Also, hepatic cytosol from sea turtles was applied on a Sephadex G-75 column and elution profiles of trace elements were examined. Copper, Zn, Se, Rb, Ag and Cd were largely present in cytosol in the liver of both species, indicating that cytosol was the significant site for the accumulation of these elements in sea turtles. In contrast, Mo and Pb were accumulated specifically in nuclear and mitochondrial fraction and microsomal fraction, respectively. Gel filtration analysis showed that Cu, Zn, Ag and Cd were bound to metallothionein (MT) in the cytosol of sea turtles. To our knowledge, this is the first report on the association of trace elements with MT in sea turtles. |
| Keywords: | Sea turtles Trace elements Subcellular distribution Metallothionein Japan |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |